As^3＋、Cr^6＋、Ni^2＋对黑褐新糠虾毒性效应研究

Study on Toxicity of As^(3+), Cr^(6+), Ni^(2+) to Neomysis awatschensis

选取黑褐新糠虾为实验动物,采用半静态暴露法开展研究了As^(3+)、Cr^(6+)、Ni^(2+)毒性效应。急性毒性实验结果表明,As^(3+)、Cr^(6+)、Ni^(2+)对黑褐新糠虾的96 h LC50分别为92.04μg/L、233.20μg/L、537.10μg/L;相关抗氧化酶系统研究结果显示,SOD和CAT两种酶对三种重金属的暴露均呈现先诱导后抑制趋势。暴露结果显示,各处理组MDA水平显著高于对照组。综合3种重金属离子对黑褐新糠虾毒性数据,3种重金属离子毒性依次为As^(3+)>Cr^(6+)>Ni2,且氧化压力损伤被认为是其主要致毒机制之一。

In this study, Neomysis awatschensis was selected as the experimental animal, and the toxicity experiments of As^3＋, Cr^6＋ and Ni^2＋ were carried out in the semi-static system. The results showed that 96 h LC50 values of As^3＋, Cr^6＋ and Ni^2＋ towards Neomysis awatschensis in the sea water were 233.20 μg/L, 537.10 μg/L and 92.04 μg/L, respectively. The activity of SOD and CAT showed that the tendency of first inducing and then inhibiting when exposed to As^3＋, Cr^6＋ and Ni^2＋. And the level of MDA in every treatment group was significantly higher than that of control group. By synthesising the data of acute toxicity and antioxidant enzyme systems, the toxicity of As^3＋, Cr^6＋ and Ni^2＋ was arranged in a descend order as the following： As^3＋〉Cr^6＋〉Ni^2＋, and the oxidative stress could be considered as one of the main reason for the toxicity.