咪唑立宾对肾病综合征大鼠足细胞损伤的影响

The effect of mizoribine on podocyte lesion of the rats with nephrotic syndrome

目的探讨咪唑立宾(MZR)对肾病综合征大鼠足细胞损伤的影响。方法将36只雄性SD大鼠按照随机数表法分为正常对照组(A组,12只)和模型组(24只),对模型组大鼠采用尾静脉注射阿霉素(6.5 mg/kg)的方法建立肾病综合征大鼠模型,筛选出建模成功者并按照随机数表法分为B组(肾病模型组)和C组(MZR干预组),各10只。在注药后8周末采用考马斯亮蓝法检测各组大鼠24 h尿蛋白定量,苏木素-伊红(HE)染色评估肾小球形态结构,通过透射电镜观察肾脏足细胞形态。结果与A、C组相比,B组大鼠24 h尿蛋白定量较高,差异有统计学意义(P<0.05),且B组大鼠肾脏足细胞损伤程度较A、C两组重,细胞外基质沉淀聚集量较A、C两组多。结论 MZR能够减轻肾病综合征大鼠足细胞损伤程度,减少细胞外基质聚集,降低24 h尿蛋白定量,为临床应用MZR治疗肾病综合征提供了依据。

Objective To investigate the effect of mizoribine（MZR） on podocyte lesion of the rats with nephrotic syndrome. Methods Thirty -six male SD rats were randomly divided into normal control group （group A, 12 rats） and model group （24 rats）. Rat nephrotic syndrome （model group） was established by injecting the adriamycin via the tail veinous injection （6. 5 mg/ kg）, and the successful ones were randomly divided into group B （nephritic syndrome group, 10 rats） and group C （mizoribine intervention group, 10 rats）. At the end of the eighth week after injection, the content of the 24 - hour urine protein was deter- mined by using coomassie brilliant blue staining, glomerular morphology was investigated by HE dyeing, and the morphology of the podocyte was studied by using transmission electron microscope. Results Compared with the group A and C, the 24 - hour urine protein of the group B was higher, and the differences were statistically significant （ P 〈 0. 05 ）. The degree of podocyte le- sion of the group B was greater than that of group A and C, and the deposition of extracellular matrix proteins was larger than that of the group A and C. Conclusion MZR can reduce the＇ injury of the podocyte lesion and the deposition of extracellular matrix proteins, decrease the 24 - hour urine protein of the rats with nephrotic syndrome, which provides theoretical basis for MZR in nephrotic syndrome.