元宝枫油对一次性力竭运动大鼠氧化应激和炎症反应的影响

The effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats after exhaustive exercise

目的探讨元宝枫油对一次性力竭运动大鼠模型生理应激反应的影响以及探讨其保护作用的机制。方法 45只健康SD雄性大鼠根据体质量随机分为安静对照组(A组,正常饮食+每天生理盐水灌胃)、力竭组(B组,正常饮食+每天生理盐水灌胃)和预处理干预-力竭组(C组,每天元宝枫油0.21mL/kg灌胃),6周后进行1次力竭游泳运动。力竭运动后处死大鼠,测定大鼠血清中肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、肌钙蛋白T(TnT),以及B型脑钠肽(BNP)4项生理指标以反应力竭运动后各组大鼠心肌损伤情况;测定肝组织丙二醛水平、超氧化物歧化酶(SOD)以及谷胱甘肽过氧化物酶(GSH-PX)的活性以反应力竭运动后动物模型肝损伤情况;同时,对3组小鼠运动至力竭所需时间进行记录及比较。结果相对B组大鼠,C组大鼠的运动至力竭所需时间显著增加(P<0.05);C组大鼠和B的血清肌酸激酶、肌酸激酶同工酶、肌钙蛋白及B型脑钠肽均显著高于A组(P<0.05);C组大鼠血清CK、CK-MB水平显著低于B组(P<0.05),TnT和BNP略低于B,但差异无统计学意义(P>0.05)。C组大鼠肝组织丙二醛水平显著低于B组(P<0.05),且SOD及GSH-PX活性显著高于B组(P<0.05)。结论元宝枫油可提高大鼠运动耐受能力,具有抗疲劳,抗氧化损伤等作用,在控制高强度运动后的肝损伤方面具有明显作用。

Objective To explore the effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats after exhaustive exercise and provide a scientific basis for the prevention of sports injury by Acer truncatum oil.Methods 45 SPF male SD rats were randomly divided into control group（A,normal diet＋saline every day）,exhaustive group（B,normal diet＋saline daily）and exhaustive with Acer truncatum oil group（C,0.21mL/kg daily Acer truncatum oil gavage）.6weeks later,the rats performed once exhaustive swimming exercise,then the creatine kinase（CK）,Creatine kinase isoenzyme（CK-MB）,Troponin T（TnT）and Btype brain natriuretic peptide（BNP）in the serum and myocardium was tested;besides,the malondialdehyde（MDA）,superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）in liver were mensurated.Results After exhaustive exercise,compared with group B,the time to exercise and exhaustion of group C increased significantly（P0.05）;Serum CK,CK,CK-MB,TnT and BNP in group B and C were significantly higher than those in group A（P0.05）;The levels of serum CK and CK-MB in group C were significantly lower than those in group B（P0.05）;TnT and BNP were slightly lower than those in group B（P0.05）,but the difference was not statistically significant;The MDA in liver tissue of group C was significantly lower than that in group B（P0.05）,and the activities of SOD and GSH-PX were significantly higher than those of group B（P0.05）.Conclusion The Acer truncatum oil could control the myocardial injury and Oxidative stress response after exhaustive exercise.