重庆市HPV感染类型及宫颈病变情况分析

Analysis of HPV infection types and cervical lesions in Chongqing

目的研究重庆市妇女感染人乳头瘤病毒(HPV)类型及宫颈病变情况。方法选取在该院宫颈筛查,年龄16~69岁的妇女5 108例,行宫颈黏液采集,使用凯普试剂盒行HPV-16、-18、-52、-58、-51、-53、-8304、-6、-11、-66、-68、-31、-33、-39、-35、-42、-43、-44、-56、-59、-45共21型检查。回顾性分析发现其中有98例HPV高危型阳性行宫颈活检,可疑病变的进一步行免疫组织化学染色。结果 HPV阳性932例,其中HPV高危型-16、-52、-58居前三位,百分比分别是18.67%、20.5%、17.81%。HPV低危型分别有53例,百分比为1.04%。混合型感染的有207例,百分比为22.2%。回顾性分析发现共有98位行宫颈活检术,病理结果分别是:宫颈癌2例,宫颈CINⅠ级9例,宫颈CINⅡ级3例,宫颈CINⅢ级11例,宫颈慢性炎56例,可疑CINⅠ7例。宫颈病检可疑CIN进一步行免疫组织化学染色的病理结果:宫颈CINⅠ级2例,宫颈CINⅢ3级4例,宫颈慢性炎症11例。结论重庆地区HPV高危型16、52、58型感染最多,HPV高危型妇女宫颈活检,有31例宫颈病变,占总活检人数的31.6%。

Objective To investigate the distribution of human papillomavirus（HPV）genotypes in infected women from Chongqing,China.Methods Samples were obtained during routine gynecologic examinations and DNA was extracted from the samples,PCR and chip was performed to distinguish the 21 HPV subtypes（-16,-18,-52,-58,-51,-53,-8304,-6,-11,-66,-68,-31,-33,-39,-35,-42,-43,-44,-56,-59,-45）.Samples were obtained from 5 108women（16 ―69years old）,and 932 cases of HPV infection were diagnosed.Results High-risk HPV-16,-52,-58 were the most common genotypes accounting for 18.67%,20.50%,17.81%of cases,respectively.In retrospective study,98 patients with high-risk HPV infection were performed to cervical biopsy,there were Cervical cancer 2cases,cervical CINⅠ9cases,cervical CINⅡ3cases,cervical CINⅢ11cases,cervical chronic inflammation 56 cases and suspected CINⅠ7cases.Conclusion In this study,HPV-16,-52,-58 were the most common subtypes found in patients from Chongqing,and 31.6percent of women were detected with cervical lesions.