宁前胡愈伤组织诱导初探

A Preliminary Study on Callus Inducement of Ning Qianhu(Peucedanum praeruptorum Dunn)

以野生宁前胡根、茎、叶为外植体对其愈伤组织诱导进行了初步研究,以期为野生宁前胡高效快速繁殖和种质资源保护提供依据。结果表明:叶片愈伤组织诱导最佳条件为NAA 0.5mg/L+TDZ 0.05mg/L,最高诱导率为95.56%,黑暗或低光照度以及叶片远轴面接触培养基有利于叶片愈伤组织的形成,岀愈最早时间为8d;茎段愈伤组织诱导最佳条件为NAA 0.5mg/L+6-BA 2.0mg/L,最高诱导率达80.00%;根愈伤组织诱导最佳条件为NAA 0.5mg/L+6-BA 2.0mg/L或2,4-D 2.0mg/L+KT 0.5mg/L,最高诱导率达78.89%。

The study in this paper aimed to provide the protocol for rapid propagation and germplasm resources conservation of wild Peucedanum praeruptorum Dunn, using root, stem and leaf as explants for callus induction.Results：The optimum condition for leaf callus induction was NAA 0.5 mg/L＋ TDZ 0.05 mg/L,the highest induction rate up.to 95.56 %, darkness or low light intensity and abaxial surface of leaf laid up on the medium was propitious to callus formation,8 days was the earliest time for callus induction.The optimum condition for stem callus inducement was NAA 0.5 mg/L＋6-BA 2 mg/L,the highest induction rate was 80.00%.While NAA 0.5 mg/L＋6-BA 2 mg/L or 2,4-D 2 mg/L ＋KT 0.5 mg/L was optimal for root callus induction,the highest induction rate reached 78.89%.