利鲁唑对戊四氮致癫痫大鼠海马CA3区IL-1β、TNF-α的影响

Effects of riluzole on the expressions of IL-1β and TNF-α in the hippocampus CA3 of rat with seizures induced by pentylenetrazole

目的探讨利鲁唑对戊四氮(PTZ)致癫痫大鼠海马CA3区白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)的影响。方法将45只大鼠按完全随机设计方法分为对照组、癫痫模型组(PTZ组)和利鲁唑组(Riluzole组),每组15只;造模大鼠连续给予PTZ(35 mg·kg-1·d-1)腹腔注射30 d,3次Ⅳ级或以上发作大鼠为癫痫造模成功;免疫组织化学法检测各组大鼠海马CA3区IL-1β、TNF-α的表达和分布。结果对照组、PTZ组及Riluzole组大鼠的IL-1β阳性细胞平均光密度值(OD)分别为(0.210±0.002)、(0.241±0.002)和(0.220±0.002),不同组间比较差异均有统计学意义(P<0.05);TNF-α阳性细胞OD值分别为(0.191±0.003)、(0.230±0.003)和(0.202±0.003),不同组间比较差异均有统计学意义(P<0.05);大鼠海马CA3区IL-1β与TNF-α表达水平呈显著正相关(r=0.969,P<0.01)。结论利鲁唑通过抑制癫痫大鼠脑内神经细胞过度表达IL-1β、TNF-α,可能降低其对正常神经细胞的损伤作用,可能减缓癫痫发病对正常神经细胞的损伤,产生神经保护作用。

Objective To investigate the effects of riluzole on interleukin-1 beta（IL-1β） and tumor necrosis factor alpha（TNF-α） levels in the hippocampus CA3 of Rat, using pentylenetetrazole（PTZ） kindling epileptic model.Methods A total of 45 rats were divided into 3 groups： the control group, the epilepsy model group（PTZ group） and the riluzole group according to the completely randomized design method, with 15 rats in each group. The model rats were continuously given PTZ（35 mg·kg^-1·d^-1） intraperitoneal injection for 30 days. Rats with 3 times of Ⅳ or more seizures were successfully modeled for epilepsy. The expressions and distributions of IL-1β and TNF-α of each group in hippocampus of CA3 were evaluated by immunohistochemical assay. Results The mean optical density（OD） of IL-1βpositive cells in the control group, PTZ group and riluzole group were（0.210±0.002）,（0.241±0.002） and（0.220±0.002）,respectively, and there were significant differences among them（P〈0.05）. The mean optical density（OD） of TNF-α in the control group, PTZ group and riluzole group were（0.191 ± 0.003）,（0.230 ± 0.003） and（0.202 ± 0.003）, respectively,and there were significant differences among them（P〈0.05）. There was a significantly positive correlation between the expressions of IL-1β and TNF-α in hippocampus CA3（r=0.969, P〈0.01）. Conclusion Riluzole prevent the excessive expression of IL-1 β, TNF-α from neurons in the brain of epileptic rats, reduce its damaging effects on normal nerve cells. Therefore, Riluzole act as a neuroprotector to alleviate damages of epilepsy.