3株果实采后葡萄孢属真菌ITS区rDNA序列与碳源代谢指纹图谱分析

Analysis of rDNA ITS and Carbon Metabolic Fingerprinting of Botrytis Nees Isolated from Three Fruits

从采后贮藏过程中发病的草莓、蓝莓和樱桃果实中分离到3株丝状真菌138#、233#和366#。综合形态学特征观察与ITS区r DNA序列分析结果,可确定3株真菌均为葡萄孢属病原菌(Botrytis)。分子鉴定进化树和碳源代谢聚类分析结果,都得到菌株138#与366#在一个大的分支上,而菌株233#在一个分支上。菌株138#鉴定结果与Botrytis cinerea(KJ937044.1)在同一分支上亲缘性达100%,且与366#亲缘性达99%;菌株233#鉴定结果与Botrytis elliptica(EU519207.1)在同一分支上且亲缘性达100%。95种碳源代谢指纹图谱分析的结果表明,3株葡萄孢属病原菌对吐温80、N-乙酰-D-半乳糖胺、N-乙酰-D-葡萄糖胺和苦杏仁苷等67种碳源的代谢能力相同,包括56种最适碳源、1种可利用碳源和10种不可利用碳源,Biolog系统中鉴定出3株丝状真菌均与B.cinerea Pers.BGA相近,其中菌株233#与B.cinerea的代谢指纹图谱最为相近。

Three strains 138^#,233^# and 366^# were isolated from infected strawberry,blueberry and cherry fruit. All the fungal pathogens were identified as Botrytis Nees by morphological characterization and r DNA ITS analysis. The results obtained by the analysis of both morphological characterization and r DNA ITS sequence showed that strains 138#and 366^# belonged to the same species and were closely related to B. cinerea（ KJ937044. 1）. In contrast,strain 233^# had a close phylogenetic relationship with B. elliptica（ EU519207. 1）. The biolog microbial identification system with FF microplate was applied for 95 different carbon and nitrogen sources utilization tests of three pathogens,and the result of the carbon metabolic fingerprinting showed that three strains had 67 carbon sources in common,including fifty-six optimal carbon sources,one available carbon source and ten unavailable carbon sources. In addition,metabolic profiling revealed that three fungi were all closely related to B. cinerea Pers. BGA and 233#was the nearest to B. cinerea Pers. BGA.