3个杂交水稻亲本成熟期种子内生细菌多样性研究

Diversity of Endophytic Bacterial Communities in Three Parental Seeds of Hybrid Rice(Oryza sativa L.) at Maturity Stage

通过传统微生物培养方法,对成熟期的杂交水稻亲本"深08S"、"和620S"及"16A007"种子内生细菌群落结构多样性进行分析。实验表明,处于成熟期的杂交水稻亲本种子内生细菌群落结构具有多样性。"深08S"种子内生细菌含40个分类操作单元(operational taxonomic units,OTU),分属Firmicutes、Proteobacteria、Actinobacteria及Bacteroidetes类群,其中,第一优势菌属为Rhizobium,丰度为15.83%,第二优势菌属为Bacillus,第三优势菌属为Sphingomonas和Curtobacterium。"和620S"种子内生细菌含20个OTU,分属Firmicutes、Proteobacteria及Actinobacteria类群,其中,第一优势菌属为Curtobacterium,丰度为25.33%,第二和第三优势菌属分别为Microbacterium和Pantoea。"16A007"种子内生细菌含24个OTU,分属Firmicutes、Proteobacteria及Actinobacteria类群,其中,第一优势菌属为Pantoea,丰度为35.00%,第二优势菌属和第三优势菌属分别为Micrococcus和Pseudomonas。相比处于灌浆期的水稻种子内生细菌,成熟期的水稻种子内生细菌更丰富,种类更多。由研究可见,种子内生细菌的种类随种子成熟度而变化,成熟期的水稻种子具有丰富的内生菌资源,可为开发新的植物益生菌剂奠定基础,且水稻种子内生细菌的组成与水稻品种有一定的关系。

The objective of this study was to investigate the endophytic bacterial communities in the seeds of three rice（ ＂Shen 08S＂,＂He 620S＂and ＂16A007＂） in maturity stage. Experimental results showed that the parental seeds of hybrid rice had diversity of the bacterial community structure at maturity stage.The endophytic bacterial communities in ＂Shen 08S＂included 40 OTUs,they were belonged to Firmicutes,Proteobacteria,Actinobacteria and Bacteroidetes,among them,the abundance of the first dominant bacterium Rhizobium was 15. 83%,the second and third dominant bacterium was Bacillus,the third dominant bacterium was Sphingomonas and Curtobacterium; The endophytic bacterial communities in＂He620S＂included 20 OTUs,they were belonged to Firmicutes,Proteobacteria and Actinobacteria,the abundance of the first dominant bacterium Curtobacterium was 25. 33%,the second and third dominant bacterium was Microbacterium and Pantoea, respectively; the endophytic bacterial communities in＂16A007＂included 24 OTUs,belong to Firmicutes,Proteobacteria and Actinobacteria,the abudance of the first dominant bacterium Pantoea was 35. 00%,the second and third dominant bacterium was Micrococcus and Pseudomonas,respectively. From this study,we found that the endophytic bacteria species in the seeds changed with seed maturation,and the seeds in maturity stage were richer in endophytes resources,which can lay the foundation for the development of new microbial inoculants,and the endophytic bacterial community structure has certain relevance in the seeds of hybrid rice which are genetically related.