产前监测血清脂氧素A4和热休克蛋白70水平对诊断子痫前期的临床价值

Clinical value of serum LXA4 and HSP70 levels in diagnosis of pre-eclampsia

目的探讨产前监测血清脂氧素A4(LXA4)、热休克蛋白70(HSP70)水平对诊断子痫前期的临床价值。方法选取2014年6月-2016年7月在该院产科行产前检查并住院分娩的单胎妊娠孕妇128例。其中,发生子痫前期的60例孕妇作为观察组,包括:轻度子痫前期组27例,早发型重度子痫前期组20例,晚发型重度子痫前期组13例。正常妊娠的68例孕妇作为对照组。分娩前抽取空腹血,采用酶联免疫吸附测定法(ELISA)检测各组孕妇血清中LXA4、HSP70表达水平,并进行对比分析。结果轻度及重度子痫前期组孕妇血清LXA4水平均低于对照组,差异有统计学意义(P<0.05),且轻度子痫前期组孕妇血清LXA4水平高于重度子痫前期组(P<0.05),早发型重度子痫前期组孕妇血清LXA4水平与晚发型重度子痫前期组比较,差异无统计学意义(P>0.05);轻度及重度子痫前期组孕妇血清HSP70水平均高于对照组,差异有统计学意义(P<0.05),轻度子痫前期组孕妇血清HSP70水平低于重度子痫前期组(P<0.05)。结论子痫前期患者产前血清LXA4水平降低、HSP70水平升高,且疾病程度越重,血清LXA4水平越低、HSP70水平越高,联合监测血清LXA4、HSP70水平对诊断子痫前期具有较高的应用价值。

Objective To explore the clinical value of serum lipoxin A4（LXA4） and heat shock protein70（HSP70） levels in the diagnosis of pre-eclampsia. Methods A total of 128 cases of singleton pregnancy scheduled for prenatal check-ups and hospital deliveries in our hospital from June 2014 to July 2016 were included in the study for retrospective data analysis. Of 60 cases with pre-eclampsia selected as the observation group, 27 cases were defined as the mild pre-eclampsia group, 33 cases were defined as severe pre-eclampsia group which included 20 cases of early-onset severe pre-eclampsia and 13 cases of late-onset severe pre-eclampsia; while 68 cases of normal pregnancy were selected from the remaining patients as the control group. Fasting blood samples were collected before delivery, the expression levels of LXA4 and HSP70 in the groups of pregnant women were detected by enzyme linked immunosorbent assay（ELISA）, and the expression levels were compared and analyzed. Results The levels of serum LXA4 in the mild and the severe pre-eclampsia group were significantly lower than those in the control group, the difference was statistically significant（P〈0.05）; and the level of serum LXA4 in the mild pre-eclampsia group was higher than that in the severe one（P〈0.05）; there was no significant difference for the levels of serum LXA4 between the early and the late onset severe pre-eclampsia group（P〈0.05）. The levels of serum HSP70 in the mild and the severe pre-eclampsia group were higher than those in the control group, the difference was statistically significant（P〈0.05）, the serum HSP70 level of the mild pre-eclampsia group was lower than that of the severe pre-eclampsia group（P〈0.05）. Conclusions The level of serum LXA4 is significantly decreased, while the level of HSP70 is significantly increased in pre-eclampsia patients. And the more severe the disease is,the lower the level of serum LXA4 is, and the higher the level of HSP70 is. Combined monitoring of serum LXA4 and HSP70 is of great value levels in the diagnosis of pre-eclampsia.