摘要
在 peroxisome 调查在 C-689T 的多型性之间的协会的目的激活 proliferator 的 receptor-2 (PPAR2 ) 倡导者和冠的心疾病(CHD ).Methods 这控制盒子的研究在 nondiabetic 汉语汉人被进行，它与 CHD (案例) 注册了 455 个病人，没有 CHD (控制) ， 693 使遭到。临床的索引的数据被收集，包括高度，身体重量，腰情形，收缩血压(SBP ) ，心脏舒张的血压(DBP ) ，吸烟、喝的、物理活动，以及身体质量索引(BMI ) 。Fasting 血葡萄糖(FBG ) ，血浆总数胆固醇(TC ) 和 triglyceride (TG ) 铺平被测量。聚合酶链限制反应的碎片长度多型性(PCR-RFLP ) 被用来决定 PPAR2 倡导者 C-689 T 替换。PPAR2 倡导者 C-689T，突变而产生之遗传的频率，临床的索引，和实验大小的遗传型分发在二个组之间被比较。 CHD 的风险上的遗传型的效果用 univariate 和在 PPAR2 倡导者 C-689T 的 CC ， CT 和 TT 的遗传型频率是的 multivariate 回归 model.Results 被估计89.7%，9.9%和0.4%在情况中组织，并且93.1%，6.6%和0.3%在控制组织，分别地( CC 对 CT+TT ，<sup>2</sup>= 6.243 ， P=0.041 )。689T 等位基因(n=95 ) 的搬运人比非搬运人(n=1053 ) 有显著地更高的 TC 水平(5.12 敦敲据 ? 漠 ? 档楯散 ? 潦汬睯摥戠 ? 桴 ? 慰楴湥獴攠慶畬瑡潩 ? 景椠普牯慭楴湯朠瑡敨敲 ? 牦浯琠敨物漠湷爠獥慥' ?湡 ? 扯敳癲瑡潩獮漠 ?瑯敨獲猠' 咨
Objective To investigate the association between the polymorphism of C-689T in the peroxisome proliferator-activated receptor-γ2 （PPARγ2） promoter and coronary heart disease （CHD）. Methods This case-controlled study was conducted in nondiabetic Chinese Han people, which enrolled 455 patients with CHD （cases） and 693 subjects without CHD （controls）. Data of clinical indexes were collected, including height, body weight, waist circumstance, systolic blood pressure （SBP）, diastolic blood pressure （DBP）, smoking, drinking, physical activity, as well as body mass index （BMI）. Fasting blood glucose （FBG）, plasma total cholesterol （TC） and triglyceride （TG） levels were measured. Polymerase chain reaction-restricted fragments length polymorphism （PCR-RFLP） was used to determine the PPARγ2 promoter C-689→T substitution. The genotype distribution of PPARγ2 promoter C-689T, allelic frequency, clinical indexes, and laboratorial measurements were compared between the two groups. The effect of genotype on the risk of CHD was assessed using univariate and multivariate regression model. Results The genotype frequencies of CC, CT and TT in PPARγ2 promoter C-689T were 89.7%, 9.9% and 0.4% in the case group, and 93.1%, 6.6% and 0.3% in the control group, respectively （CC vs. CT＋TT, χ^2= 6.243, P=0.041）. Carriers of -689T allele （n=95） had significantly higher TC level than non-carriers （n=1053） （5.12±1.26 vs. 4.76±1.22 mmol/L, P=0.001）. Male carriers of -689T allele （n=51） were significantly higher in waist circumference, body weight, TC and TG than male non-carriers （n=656） （all P〈0.05）. In subjects whose BMI was over 25 kg/m2, carriers of -689T allele （n=82） had significantly higher levels of waist circumference, BMI, SBP and TC than non-carriers （n=231） （all p〈0.05）. The -689T allele was an independent risk factor for CHD （OR=1.668, 95%CI： 1.031-2.705, P=0.037） after adjusting for age, gender, waist circumference, body weight, BMI, smoking, physical activities, SBP, DBP, FBG, TC and TG level. Conclusion These data support the hypothesis that the -689T allele is associated with an increased risk of CHD, in Chinese Han people and correlates significantly with the profiles of CHD-related risk factors.
