响应面法优化肉苁蓉松果菊苷肠溶微球的制备

Optimization of the preparation process for echinacoside in Herba Cistanche enteric microspheres by response surface methodology

目的制备肉苁蓉松果菊苷肠溶微球,筛选最佳制备工艺,并考察其体外释放特性。方法用离子凝胶-干燥法制备肉苁蓉松果菊苷肠溶微球,以包封率为考察指标,用响应面法优化其制备工艺。结果制备肉苁蓉松果菊苷肠溶微球最佳工艺参数:海藻酸钠浓度36.33mg/ml、氯化钙浓度10.82mg/ml、壳聚糖浓度10.93mg/ml。结论优选的工艺稳定、可行,制备的微球包封率高、缓释效果较好。

Objective To prepare Herba Cistanche enteric release microspheres, optimize the preparation process and study the releasing characteristics of microspheres in vitro.Methods Ion gelatin-oven drying method was used to prepare Herba Cistanche enteric microspheres.The preparation process was optimized with the aid of a Box-Behnken design.Results The optimal preparation condition was 36.33 mg/ml of sodium alginate, 10.82 mg/ml of calcium chloride and 10.93 mg/ml of chitosan.Conclusion This technology is repeatable and feasible.The microspheres have high entrapment efficiency and good sustained release characteristics.