慢性自发性荨麻疹患者血液嗜酸性粒细胞NK1R水平增加

Up-regulated expression of NK1R in eosinophil-enriched blood cells from patients with chronic spontaneous urticaria

目的检测慢性自发性荨麻疹(CSU)患者外周血嗜酸性粒细胞富集群中P物质(SP)和神经激肽/速激肽受体1(NK1R)的表达。方法收集CSU患者和健康人外周血,用(0.1、1)μg/m L蒿草花粉、尘螨和梧桐花粉过敏原粗提液刺激,流式细胞术检测嗜酸性粒细胞富集群中SP及NK1R的表达。结果与健康人相比,CSU患者嗜酸性粒细胞比例增加1.2倍,NK1R+嗜酸性粒细胞上调66%,而SP+嗜酸性粒细胞的比例下调40%。0.1μg/m L尘螨过敏原可诱导CSU患者NK1R的表达上调1.11倍。结论 CSU患者嗜酸性粒细胞NK1R表达上调,阻断NK1R可用于CSU的治疗。

Objective To investigate the expressions of substance P（ SP） and neurokinin-1 receptor（ NK1R） in eosinophil-enriched blood cells from patients with chronic spontaneous urticaria（ CSU）. Methods Peripheral venous blood samples were collected from patients with CSU and healthy controls（ HCs）,and then stimulated with crude extracts of Artemisia pollen,dust mite,and Platanus pollen（ all at concentrations of 0. 1 and 1. 0 μg/m L）. The expressions of SP and NK1 R in eosinophil-enriched blood cells were detected by flow cytometry. Results Compared with HCs, eosinophil proportion in peripheral blood of CSU patients increased 1. 2-fold. Percentage of NK1R＋eosinophils in the patients with CSU was elevated up to 66% compared with HCs when cultured in the medium only. However,the level of SP decreased by 40%in the CSU patients. In eosinophil-enriched blood cells from the CSU patients,the crude extract of dust mite at 0. 1 μg/m L induced approximately 1. 11-fold increase of NK1 R expression. Conclusion Expression of NK1 R increases in the eosinophils of CSU patients. Blockers of NK1 R might be used for CSU treatment.