摘要
目的:人参皂苷Rg1可以抑制心肌肥厚,本研究主要探讨人参皂苷Rg1对异丙肾上腺素(Isoproterenol,ISO)诱导肥大心肌细胞凋亡的抑制作用及其可能的作用机制。方法:H9C2心肌细胞传代后,以10μmol/L的ISO诱导心肌细胞肥大和凋亡,鬼笔环肽染色测定细胞体积;Lowry法测定心肌细胞蛋白含量;JC-1染色观察各组线粒体膜电位的变化;Western Blot测定bcl-2,bax和calpain-1的蛋白表达;Fluo-3/AM为荧光探针,测定细胞内钙的变化。结果:人参皂苷Rg1(40,80μmol/L)可降低ISO诱导的细胞体积增大,蛋白合成增加,增加ISO诱导的线粒体膜电位降低,增加bcl-2蛋白表达,降低bax蛋白表达。机制研究显示人参皂苷Rg1可同时降低ISO诱导的calpain-1表达增加和细胞内钙增加。结论:人参皂苷Rg1可通过Ca2+/calpain-1通路减轻ISO诱导的肥大心肌细胞凋亡。
Objective： To investigate the anti-apoptotic effect of ginsenoside Rg1 on isoproterenol（ ISO） induced hypertrophic cardiomyocyte and the underlying mechanism. Methods： Hypertrophic and apoptotic cardiomyocytes were induced by 10 μmol/L of ISO. The cell size was observed by rhodamine-labeled phalloidin staining; the total protein content was measured by the method of Lowry; the mitochondrial membrane potential（ MMP） was determined by JC-1 staining; expressions of bax,bcl-2,and calpain-1 were measured by Western Blot; Intracellular Ca2 ＋was measured by cell-loading Furo-3/AM. Results ： Pretreatment of ginsenoside Rg1（ 40,80 μmol/L） decreased cell size,protein content,and bax expression,and increased MMP,bcl-2 expression compared with ISO treatment alone. In the mechanism study,results indicated that ginsenoside Rg1 decreased the calpain-1 expression and intracellular Ca^（2＋） compared with ISO treatment alone. Conclusion： Ginsenoside Rg1 could attenuate the apoptosis of hypertrophic cardiomyocyte induced by ISO via Ca^（2＋）/calpain-1 signaling pathway.
出处
《中药药理与临床》
CSCD
北大核心
2017年第4期17-20,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
辽宁省大学生创新创业训练计划项目(201610160000058)
