Shank1在肺癌中的表达及临床意义

Expression and clinical significance of Shank1 in lung cancer

目的检测Shank家族成员Shank1在原发性肺癌中的表达，探讨在肺癌组织与癌旁组织中Shank1的表达差异，分析其与肺癌临床病理特征的关系。方法收集肺癌术后患者的癌组织与癌旁组织标本，采用免疫组化及Western blot检测Shank1的蛋白表达水平，采用RT-PCR检测Shank1的mRNA表达水平，分析Shank1表达与临床病理特征的关系。结果肺癌的癌组织中Shank1表达率高于癌旁组织（Х^2=10．176，P〈0．05）。Shank1在肺癌中的敏感度与特异度分别为56．25％和66．67％。Western blot与RT—PCR检测发现肺癌的癌组织中Shank1蛋白和mRNA表达水平明显高于癌旁组织。在癌组织中Shank1的高表达与患者的性别、年龄、病理分级、病理类型、淋巴结转移无显著相关，但与肺癌的T分期显著相关（Х^2=5．268，P〈0．05）。结论Shank1在肺癌组织中异常表达，与肺癌的T分期相关，且敏感度与特异度均较高，提示Shank1可能参与肺癌的发生、发展。

Objective To detect the expression level of Shank1 in lung carcinoma, investigate the difference of Shank1 in the tumor and paracancerous tissues, and analyze the relationship of shankl with the elinicopathologic characteristics of lung cancer. Methods The lung cancer tissues and paracancerous tissues of 96 patients were selected. The expression level of Shank1 was detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction （RT-PCR）. Statistical analysis was performed to determine the relationship between the expression of Shank1 and the clinicopathologic characteristics of lung cancer patients. Results The expression rate of Shank1 in lung cancer tissues was significantly higher than that in the paracancerous tissues （ Х^2= 10. 176, P d0.05）. Sensitivity and specificity of Shank1 were 56.25%and 66.67%. Western blot and reverse transcription- polymerase chain reaction results showed that the expression level of Shank1 in lung cancer tissues was also significantly higher than that in the paracancerous tissues. The high expression level of Shank1 in lung cancer tissues was not significantly related to gender, age, pathological grade and classification, but was significantly associated with T stage （ Х^2= 5. 268, P 〈0.05）.Conclusions Shank1 is abnormally expressed in lung cancer tissues, and is correlated with T stage. In addition, both the sensitivity and specificity of Shank1 are high.