近五年发表文献中小鼠采血方法的分析研究

The Study on Blood Collection Method in Mouse in China Journal Reports near 5 Years

目的了解国内小鼠采血方法使用的情况。方法万方数据库中检索2012至2016年间正文中含有检索词"小鼠"和"采血"的文献,对符合条件的文章进行统计分析。结果检索到448篇文献,其中符合条件的文献444篇。使用比例最高的3种采血方法依次是眼眶后静脉丛采血(33.3%)、摘眼球采血(22.6%)和尾静脉采血(15.4%)。心脏采血使用麻醉的占60%,眼眶静脉丛采血使用麻醉的占3.2%。结论摘眼球采血法的使用率很高,但鉴于其会给小鼠带来巨大痛苦,故建议用下颌静脉丛采血等其他方法替代。心脏采血、眼眶后静脉丛采血过程中实施麻醉的比例不高,应加以重视和规范。

Objective To investigate the domestic situation of method of blood collection in mouse. Method The data was retrieved from Wan Fang database in the search term ＂mouse＂and ＂blood＂ as defined by the 2012 to 2016 Journal Citation Reports. Perform a statistical analysis on the articles which meet the criterion. Result Four hundred and forty-eight articles were retrieved, and 444 articles met the criterion. The most used three blood collection method are orbital sinus （33.3%） , picking eyeball （22.6%） and tail vein （15.4%）. Heart blood with anesthesia was 60 percent, and orbital sinus blood with anesthesia was 3.2 percent. Conclusion The utilization rate of picking eyeball method is high. Given that it can cause great pain in mice, we suggest that replace this method with jaw venous plexus in pursuance or other method . The rate of heart blood and orbital sinus with anaesthesia is not high. So it should be taken into account.