参竹精片对小鼠的抗疲劳及抗氧化作用研究

Study on Anti-fatigue and Anti-oxidant Effects of Shenzujing Tablets in Mice

目的观察参竹精片的抗疲劳及抗氧化作用。方法选择120只昆明小鼠,随机分为正常对照组、参竹精片0.5 g/kg、0.25 g/kg、0.125 g/kg组和六味地黄丸对照组,用不同剂量的参竹精片灌胃治疗7 d,正常对照组给予等量生理盐水,各组于末次给药后2 h,随机选其中50%小鼠,尾根部负荷2 g铅丝,观察小鼠的力竭游泳时间;另50%小鼠尾根部负荷0.4 g铅丝,测定心肌和肌肉的SOD、MDA含量。另选择144只昆明小鼠分组和给药方法同上,各组于末次给药后,随机选其中50%小鼠,1次性灌胃给予50%乙醇0.4 m L/只,测定酒精性肝损伤小鼠血清和肝组织的MDA、SOD含量;另50%小鼠取大脑组织制成10%脑匀浆,加入Fe^(2+)-Vit C溶液,测定脑组织MDA、SOD含量。结果参竹精片能延长小鼠力竭游泳时间(P﹤0.05),提高心肌SOD活力(P﹤0.05),降低肌肉MDA含量(P﹤0.05);降低酒精性肝损伤小鼠血清和肝组织MDA含量(P<0.01),增加SOD含量(P<0.01);减少脑损伤小鼠MDA含量(P<0.01)。结论参竹精片通过抗氧化作用,能够提高心肌、肌肉和肝组织的能量代谢,具有较好的抗疲劳作用。

Objective To explore anti-fatigue and anti-oxidant effects of Shenzujing tablets in mice. Method One hundred and twenty mice were randomly divided into 5 groups： control groups, Liuwei Dihuang pill group and shenzujing tablets （0.5,0.25,0. 125 g/kg） groups. Each study group contained twenty-four mice. The mice received daily oral reatments of drugs for 7 days. The control group received daily oral treatments of isovolumic distilled water for 7 days. The 2 h after the last treatment, 50% of mice tail root were randomly selected to load 2 grams of lead wire, the exhaustion swimming time were observed. The other 50% of the mice tail root were loaded 0.4 grams of lead wire, after 1 hour swimming, SOD and MDA content in myoeardium and muscle were detected. Another 144 mice were randomly divided into 6 groups： control group, model group, Liuwei Dihuang pill group and Shenzujing tablets （0. 5,0. 25,0. 125 g/kg） groups; The administration method were similar as above. After the last treatment, 50% of mice were randomly selected and given intragastrie administration of 0.4 mL/50% ethanol, serumand liver MDA, SOD content were determined, brain tissue of the other 50% mice were made into 10% brain homogenate and put Fe2~ - VitC solution in, hatched for 20 rains at temperature of 37 ~C , brain MDA, SOD content were determined. Result The mouse swimming exhaustion time in Shenzujing tablets （0.25,0. 125 g/kg） groups were significantly prolonged （P 〈 0.05 ） , and the myocardial SOD activity were significantly improved （ P 〈 0.05 ）. The MDA contents in biceps femoris muscle were lowered （P 〈 0.05 ）. serum and liver MDA concentration in alcoholic liver injury mice were lowered （ P 〈 0.01 ） , SOD concentration were increased （P 〈 0.01 ） ; MDA content in mice of the brain injury induced by Fe2＋-VitC were reduced（P 〈 0.01 ）. Conclusion The shenzujing tablets can increase SOD activity and decrease MDA accumulation and have the ability to maintain the body＇s metabolism, increase the mice swimming exhaustion time, good anti-fatigue effect, its effect is closely related to the anti oxidant effect.