解毒化瘀方对兔颈动脉球囊损伤后内膜增殖抑制作用的研究

Inhibitive Effects of Jiedu Huayu Formula on Neointimal Hyperplasia after Carotid Artery Balloon Injury in Rabbits

目的通过建立兔颈动脉球囊损伤模型,应用解毒化瘀方灌胃治疗,观察解毒化瘀方对新生内膜增殖抑制的效果。方法将20只新西兰大白兔随机分为实验组和对照组,建立右侧颈动脉球囊损伤模型,实验组和对照组分别给予中药汤剂解毒化瘀方及生理盐水灌胃。28 d后取颈动脉标本,比较两组新生内膜厚度,新生内膜面积/中膜面积比值,以及行增殖细胞核抗原(PCNA)染色比较平滑肌细胞增殖程度。结果实验组新生内膜厚度、新生内膜面积/中膜面积分别明显小于对照组,差异有统计学意义(P<0.05)。实验组新生内膜及中膜PCNA染色阳性细胞比例均明显低于对照组,两组比较差异有统计学意义(P<0.05)。结论解毒化瘀方能明显抑制兔颈动脉球囊损伤后血管平滑肌细胞增殖效应,抑制新生内膜的形成,减轻血管内再狭窄的程度。

Objective To observe the inhibitive effects on neointimal hyperplasia in the treatment of lavage with jiedu huayu formula through setting up the model of carotid artery balloon injury in rabbits. Methods Twenty New Zealand white rabbits were randomized into an experiment group and a control group. The models of balloon injuryof the right carotid artery were established. Jiedu huayu formula and physical saline were used respectively in the lavage in the experiment group and the control group. In 28 days,the sample of carotid artery was collected. The neointimal thickness and the ratio of neointimal area and tunica medial area were compared between the two groups. The staining test with the proliferating cell nuclear antigen（ PCNA）was used to make the comparison of the proliferation of smooth muscle cell. Results Theneointimal thickness and the ratio of neointimal area and tunica medial area in the experiment group were lower apparently than those in the control group,indicating the significant differences（ P〈 0. 05）. The proportion of PCNA stained positive cells between neointima and tunica media in the experiment group was lower obviously than that in the control group,indicating the significant difference（ P 〈0. 05）. Conclusion Jiedu huayu formula inhibits obviously the proliferation of vascular smooth muscle cells and the neointimal formation and alleviates the re-stenosis of vessels.