不同方法炮制的熟地黄的补血作用比较

Comparison of Enriching Blood Effect of Rehmanniae Radix Praeparata Processed by Different Methods

目的:比较不同方法加工炮制的熟地黄饮片的补血作用。方法:采用环磷酰胺和盐酸苯肼结合法进行血虚模型的建立,分别设立空白组、模型组、阿胶补血口服液组、传统酒炖组和产地加工饮片炮制一体化组,每组给药体积均为0.02 mL·g^(-1),后三者的给药剂量分别为10 mL·kg^(-1),3.75,3.75 g·kg^(-1)。以全血外周血象、脏器指数和血清中促红细胞生成素(EPO)含量为指标对不同方法加工的熟地黄的补血作用进行综合评价。结果:与模型组相比,各给药组红细胞数目均较显著性升高(P<0.01);产地加工饮片炮制一体化组较显著性升高白细胞和血红蛋白(P<0.01),EPO(P<0.01)恢复至正常水平;传统酒炖组显著升高白细胞(P<0.05),EPO(P<0.05)恢复至正常水平;产地加工饮片炮制一体化组显著升高了胸腺指数(P<0.01),传统酒炖组明显升高了胸腺指数(P<0.05)。与空白组相比,模型组小鼠各指标均呈现显著性差异;与阿胶补血口服液组相比,产地加工饮片炮制一体化组明显升高了血红蛋白(P<0.05);与传统酒炖组相比,产地加工饮片炮制一体化组血红蛋白较显著性升高(P<0.01)。结论:不同方法加工的熟地黄饮片均有一定的补血作用,产地加工饮片炮制一体化组的熟地黄饮片略优于传统酒炖组,且该加工工艺具有一定的可行性。

Objective： To compare the different effect of Rehmanniae Radix Praeparata processed by different methods on the chemical damage anemia in mice as model of blood deficiency. Method ： Blood deficiency model were induced by phenylhydrazine hydrochloride and cyclophosphamide. The experimental animals were randomly divided into blank group, model group, Ejiao blood enriching oral liquid group, traditional wine stewing group and origin processing integration group. The administration volume was 0.02 mL·g^-1, and dose of the latter three was 10 mL· kg^-1, 3.75, 3.75 g·kg^-1, respectively. Enriching blood effect of Rehmanniae Radix Praeparata processed by different methods was evaluated by taking hemogram, level of erythropoietin （EPO） in serum and organ index as indexes. Result： Compared with the model group, red blood cells （RBC） of each administration group increased significantly （P 〈 0.01 ） ; white blood ceils （WBC） and hemoglobin （HB） of origin processing integration group increased significantly （P 〈 0.01 ） , EPO was gradually restored to normal level and significantly decreased （P 〈0.01 ）; WBC was increased in the traditional wine stewing group （P 〈 0.05）, while EPO （P 〈 0.05 ） returned to normal levels; Thymus index of origin processing integration group was significantly increased （P 〈 0.01 ） and the parameter in traditional wine stewing group was increased （P 〈 0.05）. Compared with the blank group, these indexes of model group were significantly different. Compared with the Ejiao blood enriching oral liquid group, HB （P 〈 0.05 ） was significantly increased in the origin processing integration group. Compared with the traditional wine stewing group, HB of the origin processing integration group was significantly increased （P 〈 0.01 ）. Conclusion： Rehmanniae Radix Preparata processed by different methods has a certain blood enriching effect. Among them, the origin processing integration group is better than the traditional wine stewing group.