开心散乙醇提取物中远志皂苷的入血成分及体内代谢产物分析

Identification of Polygala Saponins and Their Metabolites in Vivo from Ethanol Extract of Kaixinsan

目的:鉴定开心散60%乙醇提取物(KXS-60%E)及其血中移行成分中的远志皂苷类成分,分析大鼠体内远志皂苷类成分的代谢产物。方法:应用UPLC-TOF-MS技术,分析TOF-MS/MS给出的二级离子信息,结合液质联用工作站Mass Lynx v4.1分析软件及现有的文献数据库,鉴定KXS-60%E中远志皂苷类成分、灌服KXS-60%E后血清中的远志皂苷类成分及其代谢产物。结果:鉴定了KXS-60%E中14个远志皂苷类成分,主要为三萜型皂苷;鉴定了大鼠口服KXS-60%E后血中的5个原型皂苷类成分及4个代谢产物。结论:远志皂苷类成分及4个代谢产物可能是KXS-60%E潜在的活性成分及直接药效物质基础,这将有助于阐明开心散防治阿尔兹海默症的有效成分及作用机制。

Objective： Polygala saponins in the 60% ethanol extract of Kaixinsan （KXS-60% E） were identified, and the polygala saponins as well as their metabolites were analyzed in rat plasma after oral administration of KXS-60% E. Method： Ion fragments data of polygala saponins in KXS-60% E was identified by UPLC-TOF-MS. Similarly, the polygala saponins and their metabolites were also identified in rat plasma after oral administration of KXS-60% E with the ion fragments data, MassLynx v4.1 software and databases in the literatures. Result： A total of 14 polygala saponins which were mainly triterpene saponins, were identified in KXS-60% E in vitro. Moreover, 5 polygala saponins as well as 4 metabolites were identified in rat plasma. Conclusion： Five polygala saponins as well as four metabolites may be the potential bioactive compounds and direct pharmcodynamic basis of KXS-60% E. Further study on these constituents may find out the effective compounds in KXS-60% E and clarify the mechanism on treatment of Alzheimer＇s disease.