GSTP1表达下调促进膀胱癌细胞T24凋亡

GSTP1 down-expression promotes the apoptosis of bladder cancer T24 cell

目的通过慢病毒表达系统,使用shRNA下调人膀胱癌T24细胞GSTP1基因的表达,研究其对T24细胞增殖的影响,并探讨其可能的作用机制。方法通过慢病毒表达系统,建立稳定低表达GSTP1基因的T24细胞株;进行CCK-8实验以及克隆形成实验,检测GSTP1表达下调对T24细胞增殖的影响;对细胞进行Annexin V-FITC/PI染色,使用流式细胞仪,检测GSTP1表达下调对T24细胞凋亡的影响。结果与未处理组、对照组相比,GSTP1干扰组的T24细胞增殖受到明显抑制,差异有统计学意义(P<0.01);细胞凋亡检测结果显示,GSTP1表达下调后明显促进T24细胞凋亡,差异有统计学意义(P<0.01)。结论 GSTP1表达下调抑制膀胱癌细胞T24增殖,并其机制可能与促进T24细胞凋亡相关。

Objective To investigate the effect of GSTP1 down-expression on the proliferation and apoptosis of hu- man bladder cancer cell T24. Methods The GSTP1 shRNA lentivirus obtained from 293T cells which were cotrans- letted with lentiviral vector GSTP1 shRNA, pA2 and pMDG2 by Lipofectamine 2000. The infected T24 was divided into untransfected group, sh-control group and sh-GSTP1 group. Cell prolifel＇ation assays （cck-8）, clonogenic as- says and Annexin V-FITC/PI staining were used to detected the function of GSTP1 down-expression on the prolifer- ation and apoptosis of T24 respectively. The changes of GSTP1 protein level were detected by Western blot. Results Cell proliferation was inhibited remarkably（P 〈0. 01 ） after GSTP1 down-expression in T24 Cells. The rate of cell apoptosis was obvious higher than that in sh-control group and the untransfected group. Conclusion GSTP1 down-expression inhibits cell proliferation and promotes the apoptosis of T24 cell.