Twist1在食管癌中的表达及其临床意义研究

Expression of Twist1 in esophageal carcinoma and its clinical significance

目的研究Twist1在食管鳞状细胞癌中的表达情况,探讨其在食管癌演变的过程中的作用及临床意义。方法用Western blot和免疫组织化学法检测正常食管组织、食管癌旁组织(距癌组织2 cm内组织)、食管鳞癌组织中Twist1的表达情况并阐明其与性别、年龄、肿瘤位置、细胞分化程度、临床分期、T分期以及有无淋巴结转移等的关系。结果Twist1在食管鳞癌组织中阳性表达率(74.7%)明显高于癌旁组织(49.3%)和正常食管组织(38.4%),差异有统计学意义(P=0.000)。食管鳞状细胞癌中Twist1的阳性表达率在不同细胞分化程度、临床分期、T分期组间差异有统计学意义(P<0.05),在不同性别、年龄、肿瘤部位、有无淋巴结转移组间差异无统计学意义。正常食管组织组、癌旁组织组、食管鳞癌组织组间Twist1的相对表达量差异有统计学意义(F=6.263,P=0.002)。结论 Twist1可能参与了食管鳞状细胞癌的发生、发展及恶性转化过程,检测Twist1有助于食管鳞癌的诊断、判断其恶性程度。

Objective To study Twistl expression in esophageal squamous cell carcinoma, and to explore its role and clinical significance in the process of esophageal cancer. Methods Immunohistochemical method and Western blot were used to detect Twistl expression in normal esophageal tissue, para-carcinoma tissue （from cancer organi- zations within 2 cm） and esophageal squamous carcinoma tissues, and elucidated its relationship with gender, age, tumor location, degree of cell differentiation, clinical stage and lymph node metastasis. Results The positive ex- pression rate of Twistl in esophageal squamous carcinoma tissue（74.7% ） was obviously higher than that in para- carcinoma tissue （49.3 % ） and normal esophageal tissue （38.4%） （P = 0. 000）. The positive expression rate of Twistl in esophageal squamous cell carcinomas of their different cell differentiation, clinicalstage, T stage between the groups, the difference was statistically significant （ P 〈 0.05 ）, there was no statistically significant difference between the different gender, age, tumor location and lymph node metastasis groups. Relative expression levels of Twistl difference between groups of normal esophageal tissue, para-carcinoma tissue and esophageal squamous car- cinoma tissues was statistically significant （F = 6. 263, P = 0. 002）. Conclusion Twistl may be involved in the occurrence and development and malignant transformation process of esophageal squamous cell carcinoma, detection of Twistl contributes to the diagnosis of esophageal squamous cell carcinomas, and help to determine its malignant degree.