晚期肺癌外周血调节T细胞及CD8+T细胞表面PD-1表达的关系

Correlation between peripheral blood regulation of T cells and expression of PD-1 on CD8+T cells surface in advanced lung cancer

目的探讨晚期肺癌患者外周血中淋巴细胞亚型表面程序性死亡受体1(PD-1)的表达水平。方法 30例晚期肺癌患者,采用流式细胞仪检测外周血中CD4+、CD8+、CD4+/CD8+、CD4+CD25+T细胞(调节性T细胞,Tregs)及CD8+T细胞表面PD-1(CD8+CD279+T)百分比,并与30例健康人的血液标本进行对比。结果与健康人比较,晚期肺癌患者CD4+及CD4+/CD8+T细胞水平有所降低,但差异无统计学意义(P>0.05);晚期肺癌患者CD8+T细胞水平为(27.70±12.87)%,CD8+CD279+T细胞水平为(2.03±1.35)%较健康人的(22.67±5.88)%、(1.26±0.83)%显著升高,差异有统计学意义(P<0.05);晚期肺癌患者CD4+CD25+T细胞数量(2.01±1.26)%显著高于健康人(0.98±0.55)%,差异有统计学意义(P<0.05)。结论晚期肺癌外周血CD4+CD25+T细胞数量显著高于健康人。

Objective To investigate the expression level of programmed peripheral death receptor-1（PD-1） in peripheral blood lymphocyte subtype of patients with advanced lung cancer. Methods The percentage of CD4＋, CD8＋, CD4＋/CD8＋, CD4＋CD25 ＋ T cells（regulatory T cells, Tregs） and percentage of PD-1（CD8＋CD279＋T） on CD8＋T cells surface in peripheral blood of 30 patients with advanced lung cancer were detected by flow cytometry, and they were compared with 30 cases of healthy blood samples. Results Compared with healthy people, patients with advanced lung cancer had lower CD4＋, CD4＋/CD8＋T cell level, but the difference was not statistical significant（P〉0.05）. Patients with advanced lung cancer had higher CD8＋T cell level as（27.70±12.87）% and CD8＋CD279＋T cell level as（2.03±1.35）% than（22.67±5.88）% and（1.26±0.83）% in healthy people, and the different was statistically significant（P〈0.05）. Patients with advanced lung cancer had obviously higher number of CD4＋CD25＋T cell as（2.01±1.26）% than（0.98±0.55）% in healthy people, and the different was statistically significant（P〈0.05）. Conclusion The number of CD4＋CD25＋T cells in peripheral blood of patients with advanced lung cancer is significantly higher than that of healthy people.