IRAK1、TRAF6表达量与口腔扁平苔藓病灶内炎症反应、免疫应答的相关性研究

Correlation of IRAK1 and TRAF6 expression with inflammatory response and immune response in oral lichen planus lesions

目的:研究IRAK1、TRAF6表达量与口腔扁平苔藓病灶内炎症反应、免疫应答的相关性。方法:选择在本院诊断为口腔扁平苔藓的患者作为研究的OLP组,收集口腔扁平苔藓病灶组织;另取同期因口腔外伤或颌面部整形接受手术的42例患者作为研究的对照组,收集正常口腔黏膜组织。检测组织样本中IRAK1、TRAF6、TLR4信号通路分子、Th1/Th2/Treg/Th17转录因子及细胞因子的表达量。结果:OLP组患者口腔扁平苔藓病灶组织中IRAK1、TRAF6、TLR4、MyD88、NF-kB的mRNA表达量和蛋白表达量均显著低于对照组,T-bet、IFN-γ的含量均显著低于对照组,GATA3、FOXP3、RORγt、IL-4、IL-10、IL-17的含量均显著高于对照组;口腔黏膜组织中IRAK1、TRAF6的表达量与TLR4、MyD88、NF-kB的表达量以及T-bet、IFN-γ的含量呈正相关,与GATA3、FOXP3、RORγt、IL-4、IL-10、IL-17的含量呈负相关。结论:口腔扁平苔藓病灶内IRAK1、TRAF6的表达量能够抑制TLR4炎症反应通路并造成Th1/Th2/Treg/Th17免疫应答紊乱。

Objective： To study the correlation of IRAK1 and TRAF6 expression with inflammatory response and im- mune response in oral lichen planus lesions. Methods： Patients who were diagnosed with oral lichen planus in Ziyang First Peo- ple＇s Hospital were selected as the OLP group of the study, and the oral lichen planus lesions were collected; Forty-two patients who accepted surgery for oral trauma or maxillofacial plastic surgery were selected as the control group of the study, and the normal oral mucosa tissue was collected. The expression of IRAK1, TRAF6 and TLR4 signaling pathway molecules, Th1/ Th2 /Treg/Thl7 transcription factors and cytokines in tissue samples were detected. Results： IRAK1, TRAF6, TLR4, MyD88 and NF-kB mRNA expression and protein expression in oral lichen planus lesions of OLP patients were significantly lower than those of control group, T-bet and IFN-γ levels of OLP patients were significantly lower than those of control group, and GATA3, FOXP3, RORγt, IL-4, IL-10 and IL-17 levels of OLP patients were significantly higher than those of control group; IRAK1 and TRAF6 expression in oral mucosa tissue were positively correlated with TLR4, MyD88 and NF-kB expression as well as T-bet and IFN y levels, while were negatively correlated with GATA3, FOXP3, RORyt, IL-4, IL-10 and IL-17 levels. Conclusion.. IRAK1 and TRAF6 expression in oral lichen planus lesions can inhibit the TLR4 inflammatory response pathway and lead to Thl/Th2 /Treg/Thl7 immune response disorder.