纵条纹炭角菌子实体的化学成分研究

Chemical Constituents from Fruiting Bodies of Xylaria striata

目的:研究纵条纹炭角菌子实体的化学成分。方法:采用硅胶柱层析、Sephadex LH-20柱色谱及半制备型HPLC等色谱方法进行分离纯化,根据波谱数据鉴定化合物的结构。采用MTT法对化合物1~3、7进行体外肿瘤细胞毒活性测试。结果:从纵条纹炭角菌子实体的95%乙醇提取物中分离得到10个化合物,分别鉴定为:细胞松弛素D(1)、19,20-环氧细胞松弛素Q(2)、细胞松弛素Q(3)、19,20-环氧细胞松弛素D(4)、19,20-环氧细胞松弛素C(5)、反式对羟基肉桂酸乙酯(6)、吲哚-3-甲醛(7)、次黄嘌呤核苷(8)、尿嘧啶(9)、尿嘧啶核苷(10)。体外肿瘤细胞毒活性实验结果显示化合物7(40μmol/L)对HCT116、B16、U937肿瘤细胞的相对抑制率分别为57.12%、53.39%、57.85%。结论:所有化合物均首次从该菌中分离得到,其中,化合物7具有中等的肿瘤细胞毒活性。

Objective：To investigate the chemical constituents from fruiting bodies of Xylaria striata. Methods：The chemical con- stituents were separated and purified by silica gel, Sephadex LH-20 column chromatographies, and semi-preparative HPLC. Their struc- tures were determined on the spectroscopic data. The inhibitory effects of compounds 1 - 3 and 7 on cell lines were tested in vitro by MTF method. Results ：Ten compounds were isolated and identified as cytochalasin D（ 1 ）, 19,20-epoxycytochalasin Q （2）, eytochalasin Q （ 3 ） , 19,20-epoxycytochalasin D （ 4 ） , 19,20-epoxycytochalasin C （ 5 ） , trans-p-hydroxyl einnamic acid ethyl ester （ 6 ） , indole-3-carbox- aldehyde （7）, xanthosine （ 8 ） , uracil（ 9 ）, and uridine （ 10 ）. At doses of 40 μmol/L, the inhibitory rates of compound 7 on HCT116, B16 and U937 were 57.12% ,53.39% and 57.85% , respectively. Conclusion：All compounds are isolated from Xylaria striata for the first time. , and compound 7 has moderate cytotoxicity.