关于雄激素脱发毛囊隆突区的转录组学研究

RNA-seq study of the hair follicular bulge region in androgenetic alopecia

目的研究雄激素脱发患者前额区、后枕区及正常人后枕区毛囊隆突区的转录组表达差异,以探讨雄激素脱发中隆突区的病理改变机制。方法分别从10例雄激素脱发患者的前额区和后枕区以及10例正常志愿者后枕区取毛囊,经显微解剖分离获取毛囊的隆突区组织,采用第2代高通量测序技术对上述3组组织进行转录组表达差异检测分析;通过功能注释和生物信息学分析进一步探讨毛囊隆突区在雄激素脱发中的关键机制改变。结果雄激素脱发患者前额区与后枕区相比有214个差异基因;雄激素脱发患者前额区和后枕区与正常人后枕区相比分别有511个和399个差异基因。差异基因的功能分析主要富集在组织重构和DNA损伤方向。结论在雄激素脱发的发展过程中,毛囊隆突区发生了重要的病理改变,包括组织重构和DNA损伤。

Objective To explore the differencein hair follicle bulge in the frontal andoccipital areas between people with normal hair growth and patients with androgenetic alopecia （AGA）.The pathological mechanism of the hair follicular bulge region in AGA patients will be investigated.Methods Hair follicles were harvested from the frontal andoccipital areas of 10 AGA patients and 10 healthy volunteers.The hair follicular bulge was obtained through micro-dissection for further RNA-seq study.The transcription expression difference of the above tissues was detected and analyzed with second generation high-throughput sequencing technology.The key mechanisms of change in the hair follicular bulge in AGA was further exploredby function annotation and bioinformatics analysis.Results There were 214 differentially-expressed genes （DEGs） between the frontal and occipital area in the AGA patients.There were 511 and 399 DEGs in the frontal and occipital areas between the AGA patients and the healthy volunteers.The functional analysis of differential genes was mainly concentrated ontissue remodeling and DNA damage.Conclusion In the development of AGA,important pathological changes occur in the hair bulge region,including tissue remodeling and higher DNA damage.