婴儿巨细胞病毒性肝炎患儿体内一氧化氮和内皮型一氧化氮合酶水平的研究

Role of Nitric oxide and endothelial nitric oxide synthase in infants with cytomegalovirus hepatitis

目的 探讨婴儿巨细胞病毒性肝炎（ICH）患者体内一氧化氮（N0）和内皮型一氧化氮合酶（eNOS）的水平及其临床意义.方法 ELISA方法检测56例ICH患儿和50例健康对照组血清NO和eNOS的水平,统计学方法分析其与患儿肝功能、人巨细胞病毒（HCMV）DNA拷贝数的关联.结果 ICH组NO和eNOS的水平显著高于健康对照组（P〈0.01）.ICH组中ALT异常组NO和eNOS水平显著高于ALT正常组（P〈0.05）.ICH组中NO和eNOS的水平与ALT呈明显正相关（r=0.682,r=0.746,P〈0.05）,但与HCMV-DNA拷贝数无显著相关性（r=-0.087,r=-0.134,P〉0.05）.结论 ICH患儿体内NO以及eNOS水平显著升高,与患儿肝脏功能损伤呈正相关.

Objective To investigate the potential clinical role of nitric oxide （NO） and endothelial nitric oxide synthase （eNOS） in infants suffering from infantile cytomegalovirus hepatitis （ICH）.Methods NO and eNOS were measured in 56 ICH infants and 50 healthy controls by using ELISAmethod.Results NO and eNOS were significantly increased in ICH group as compared with the controls （P 〈 0.01）accompanied by increased ALT in infants of ICH group （P 〈 0.05）.Both of NO and eNOS levels were significantly positively correlated with the level of ALT（r =0.682,r =0.746,P 〈 0.05）.However,neither NO level nor eNOS level was significantly correlated with the number of copies of HCMV-DNA （r =-0.087,r =-0.134,P 〉 0.05）.Conclusions The expressions of NO and eNOS were significantly increased in infants with human cytomegalovirus hepatitis,and were closely related to the severity of liver lesion.