HCMV感染致培养脐静脉内皮细胞活化和氧化应激的作用

Role of HCMV infection in the activation and oxidative stress of cultured human umbilical vein endothelial cells

目的 初步探讨人巨细胞病毒（HCMV）感染致内皮细胞损伤的机制.方法 体外培养人脐静脉内皮细胞（HUVEC）,以HCMV AD169株感染HUVEC,24 h后通过实时定量PCR方法检测细胞粘附分子VCAM-1 mRNA的表达.通过硝酸还原酶法检测细胞培养基中一氧化氮（N0）含量的变化.结果 HUVEC感染HCMV后24 h,VCAM-1 mRNA的表达水平较对照组明显增加,两组间差异具有统计学意义（P〈0.05）.感染HCMV后0-36 h的不同时间点,感染组细胞培养上清中NO含量均较对照组增加,呈现时间依赖性,各时间点两组间差异均具有统计学意义（P〈0.01）.结论 HCMV感染可增强内皮细胞氧化应激活动,可能在血管内皮细胞活化损伤过程中起重要作用..

Objective To investigate the role of the activation and oxidative stress of cultured human umbilical vein endothelial cells （HUVEC） after HCMV infection.Methods HUVECs were divided into four groups：control,HCMV（＋）,after HCMV AD169 infection,and the supernatant of the culture was extracted,and the cells were lysed.The levels of vascular cellular adhesion molecule-1 （VCAM-1） in HUVEC were measured by real-time PCR.And the content of nitrogen nonoxide （NO） of the supernatant was detected by nitrate reductasemethod accordingly.Results Twenty-four hours after infection,the mRNA expression of VCAM-1 in HUVECs of HCMV infected group increased obviously compared to control,and NO quantity increased accordingly and time-dependently.There was significant difference between groups（P〈0.01）.Conclusions HCMV increases the mRNA expression of VCAM-1 and quantity of NO,which may contribute to the formation of AS.