新疆兵团2011-2016年肠道病毒71型分子进化特征分析

Molecular evolutionary characteristics analysis of enterovirus 71 in Xinjiang Production and Construction Corps from 2011 to 2016

目的 分析新疆兵团2011-2016年肠道病毒71型（EV71）的基因亲缘关系,为手足口病的预防控制提供病原学资料.方法 采用RT-PCR扩增标本中EV71的VP1编码区基因,进行核苷酸序列测序及序列分析.利用MEGA6.06与GenBank代表株进行核苷酸同源性分析并构建系统进化树.结果 兵团37株EV71流行株之间VP1区核苷酸和氨基酸序列同源性分别为87.3％-100.0％和93.1％ -100.0％,均为C4a基因亚型,分别处于7个相对独立的小的进化分支,流行株与代表株相比共有28处氨基酸位点发生了变异,有9株在第283氨基酸位点发生了由S（丝氨酸）到T（苏氨酸）的变异,在293氨基酸位点发生了由A（丙氨酸）到S（丝氨酸）的变异.结论 兵团2011-2016年EV71流行株均为C4a基因亚型,与代表株相比VP1区有28处氨基酸位点发生了变异,有9株流行株在283和293氨基酸位点发生了共同变异.

Objective To analyze the phylogenetic characteristics of enterovirus 71 （EV71） in Xinjiang Production and Construction Corps from 2011 to 2016,providing pathogenic information for prevention and control of hand-foot-mouth disease （HFMD）.Methods The EV71 positive strains were conducted for reverse transcription polymerase chain reactions （RT-PCR） amplification on entire VP1 coding region and sequencing was then performed and finally phylogcnetic tree was constructed among these strains and representative strains from GenBank using MEGA6.06.Results The homology of nucleotide and amino acid of the 37 EV71 strains were 87.3%-100.0% and 93.1%-100.0%,and belonged to EV71 C4a subgenotype,of 7 relatively independent small branches.Of the epidemic strains,compared with the representative strains,mutation occurred on 28 amino acid sites,the variation （Serine to Threonine,Alanine to Serine） happened on the 283 and 293 amino acid site in 9 strains.Conclusions The 37 EV71 strains in Xinjiang Production and Construction Corps from 2011 to 2016 belonged to EV71 C4a subgenotype,mutation occurred on 28 amino acid sites,and there is a common variation on the 283 and 293 amino acid site in 9 strains.