慢性间歇性低氧条件下大鼠龈沟液中牙龈卟啉单胞菌的变化

The change of Porphyromonas gingivalis in rat gingival crivicular fluid under chronic intermittent hypoxia

目的:探讨间歇性低氧条件下大鼠龈沟液中牙龈卟啉单胞菌的变化。方法:将32只6周龄雄性SD大鼠随机分成4组(n=8):常氧对照组(A组)、常氧牙周炎组(B组)、间歇性低氧组(C组)、间歇性低氧合并牙周炎组(D组)。用正畸结扎丝结扎双侧上颌第二磨牙颈部和高糖饮食方法建立牙周炎模型,常氧和低氧组分别在常氧和模拟阻塞性睡眠呼吸暂停低通气综合征条件下饲养8周,采集大鼠实验牙龈沟液标本,实时荧光定量PCR方法检测牙龈卟啉单胞菌的变化。结果:所有的大鼠均检出牙龈卟啉单胞菌。D组牙龈卟啉单胞菌的量明显高于其他3组(P<0.05);B组牙龈卟啉单胞菌的量高于A组(P<0.05)。结论:慢性间歇性低氧可加重牙周炎病程,与龈沟液中牙龈卟啉单胞菌的增加有关。

Objective： To study the change of Porphyromonas gingivalis （P. gingivalis） in rat gingival crivicular fluid （GCF） under chronic intermittent hypoxia. Methods ： 32 male SD rats were randomly divided into 4 groups （ n = 8 ） ： normoxia control group （ A）, normoxia with periodontitis group （ B）, intermittent hypoxia group （ C）, and intermittent hypoxia with periodontitis （D）. Periodontitis model was established by orthodontic silk ligation at the maxillary second molar neck and high sugar diet. The rats in normoxia and hypoxia group were raised respectively under the condition of ordinary oxygen and chronic intermittent hypoxia respectively. After 8 weeks, GCF of the target teeth was collected, P. gingivalis was quantified by real-time fluorescent quantitative PCR. Results： P. gingivalis was detected in all groups. P. gingivalis in group D was more than in other groups（P 〈 0.05 ） ; meanwhile,P, gingivalis in group B was more than in group A（P 〈0.05 ）. Conclusion： Chronic intermittent hypoxia can aggravate the severity of periodontitis, which is associated with the increase of P. gingivalis in GCF.