摘要
目的:研究透明质酸(HA)、TGF-β1因子对下颌骨髁突软骨增殖分化的影响。方法:取新生小鼠的下颌骨髁突软骨体外进行组织培养,按培养液内添加因子不同分为对照组、HA(0.5 mg/ml)、TGF-β1(5 ng/ml)组,于培养1、2、4、6、8周后进行形态学观察、软骨面积测量、茜素红染色以及碱性磷酸酶染色研究。结果:对照组中髁突软骨在培养4周后软骨内开始出现高密度光阻射区,茜素红染色、碱性磷酸酶染色提示软骨基质出现了钙化、软骨内成骨的过程;HA组中髁突软骨内未出现高密度光阻射区,而髁突软骨面积却显著增大(P<0.05);TGF-β1组中髁突软骨在培养2周后提前出现了高密度光阻射区,然软骨面积无显著改变(P>0.05)。结论:在体外培养下,HA可以促进髁突软骨的增殖,对软骨细胞的肥大分化有一定的抑制作用,TGF-β1在早期可显著促进髁突软骨细胞的肥大分化。
Objective: To study the effects Of hyaluronic acid(HA) and TGF-β1 on the growth of mandibular condylar cartilage and the hyperthophic differentiation of the condylar chondrocyts. Methods: 60 condyle samples from newborn mice were in vitro cuhured and treated with HA(0.5 mg/ml), TGF-beta 1 (5 ng/ml) and without additional agent(the control) respectively. The Morphological observation, Alizarin Red Staining, Alkaline phosphatase staining and condylar cartilage surface area measurement were conducted after 1, 2, 4, 6 and 8 weeks of culture respectively. Results: Highdensity photoresist area was observed in the condylar cartilage of the control group after 4 weeks of culture. Alizarin Red Staining and Alkaline phosphatase staining showed condylar cartilage matrix production and calcification. The HA group showed no highdensity photoresist area at all time points, however, the cartilage area was significantly increased( P 〈 0. 05 ) ; the TGF-beta 1 group showed high-density photoresist area after 2 weeks of culture, but the cartilage area were not significantly changed(P 〉 0. 05). Conclusion: HA can promote the growth of condylar cartilage in vitro, but have an inhibitory effect on chondrocyte differentiation. TGF-β1 plays a role in mandibular condylar chondrocyte hypertrophic differentiation in the early days of in vitro culture.
出处
《实用口腔医学杂志》
CSCD
北大核心
2017年第5期584-588,共5页
Journal of Practical Stomatology
基金
十二五军队重点课题(编号:BWS11J046)