miR-106a在肾癌组织中的表达及对肾癌细胞系A498增殖的影响

Expression of miR-106a in renal cancer tissues and its effect on the proliferation of renal cancer cell line A498

目的研究miR-106a在肾癌、癌旁正常组织表达情况及其在肾癌细胞系A498中的作用。方法运用real-time PCR检测33例肾癌及相应的癌旁正常组织中miR-106a的表达,分析其与临床病理特征的相关性;在肾癌A498细胞中转染miR-106a过表达载体及其抑制剂,实验分为4组:空载体对照组、miR-106a过表达载体组、阴性对照组、miR-106a抑制剂组;MTT实验检测miR-106a对肾癌A498细胞增殖的影响;应用流式细胞仪分析miR-106a对细胞周期的影响。结果 miR-106a在肾癌组织中的表达显著上调(P<0.01),并与肾癌的T分期有相关性(P<0.05)。miR-106a过表达载体与空载体对照组相比,促进了细胞增殖,G_0/G_1期细胞数量显著减少(P<0.01),S和G_2/M期细胞数量显著增加(P<0.01);而miR-106a抑制剂组与阴性对照组相比,细胞增殖抑制,G_0/G_1期细胞数量显著增加(P<0.01),S和G_2/M期细胞数量显著减少(P<0.01)。结论 miR-106a在肾癌组织中上调,通过促使细胞进入S和G_2/M期而促进肾癌细胞分裂、增殖。

Objective To investigate the expression of miR-106 a in human renal cancer tissues and its role in renal cancer cell line A498. Methods The miR-106 a expression was detected in 33 cases of renal cancer by real-time PCR. The correlation of miR-106 a expression with clinicopathological characteristics was analyzed. MiR-106 a overexpression vector and miR-106 a inhibitor were transfected into A498 cells. A498 cells were divided into four groups： empty vector control group,miR-106 a overexpression vector group,negative control group and miR-106 a inhibitor group. The proliferation of A498 cells was examined by MTT assay.The effect of miR-106 a on the cell cycle of A498 cells was observed by flow cytometer. Results The expression of miR-106 a was significantly up-regulated in renal cancer tissues（ P〈0. 01）. High miR-106 a expression was associated with T stage（ P〈0. 05）. Compared with empty vector control group,A498 cell proliferation was promoted in miR-106 a overexpression vector group,the proportion of G1/G0 phase cells was decreased（ P〈0. 01）,and the proportion of S and G2/M phase cells was increased（ P〈0. 01）. Compared with negative control,miR-106 a inhibitor suppressed A498 cell proliferation,increased the proportion of G1/G0 phase cells（ P〈0. 01）,and decreased the proportion of S and G2/M phase cells（ P〈0. 01）. Conclusion The expression of miR-106 a may increase in renal cancer tissues,and promote the division and proliferation of renal cancer A498 cells through driving cells into S and G2/M phases.