大鼠前额叶皮质miR-182与酒精依赖的关系

Relationship between expression of miR-182 in prefrontal cortex and alcohol dependence in rats

目的探讨大鼠前额叶皮质miR-182表达与酒精依赖的关系，以及可能的作用机制。方法将60只大鼠随机分为持续饮酒组、戒断组和对照组，每组各20只。用灌胃法制备酒精依赖模型，用实时荧光定量PCR检测酒精依赖大鼠前额叶皮质miR-182与BDNFmRNA的表达，并对两者的表达量进行Pearson相关分析。结果三组间miR-182表达水平的差异有统计学意义（F=44．79，P〈0．01），两两比较差异均有统计学意义（P〈0．05）。持续饮酒组与对照组比较，BDNF的mRNA表达下调，差异有统计学意义（P〈0．05）。miR-182与BDNFmRNA表达量之间无相关性（P〉0．05）。结论酒精依赖大鼠前额叶皮质miR-182表达水平下调，miR-182可能参与酒精依赖的发病过程。

Objective To study the relationship between expression of miR-182 in prefrontal cor- tex and alcohol dependence in rats and explore the possible mechanism. Methods Totals of 60 rats were randomly divided into 3 groups： continuous alcohol group （n=20）, withdrawal group （n=20）and control group （n=20）. Lavage was used to build alcohol dependence model. Real-time quantitative PCR was used to detect the expression of miR-182 and BDNF mRNA in prefrontal cortex of alcohol dependent rats. Pearson analysis was carried out to analyse the correlation between expression of miR-182 and BDNF mRNA. Results The expres- sion of miR-182 was significantly different among three groups（F=44.79, P 〈 0.01）. Significant differences were found between each two groups （P 〈 0.05）. Compared with control group, the expression of BDNF mRNA was significantly decreased in continuous alcohol group （P 〈 0.05）. The expression of miR-182 and BDNF mRNA was not correlated （P 〈 0.05）. Conclusions The expression of miR-182 in prefrontal cortex of alcohol depen- dent rats is decreased, miR-182 may be involoved in the pathogenesis of alcohol dependence.