葛根多糖的体外抗氧化活性研究

In vitro antioxidative effects of Puerariae Radix polysaccharides

目的考察葛根多糖的体外抗氧化作用。方法从葛根中提取多糖,苯酚-硫酸法测定多糖含量。分别研究葛根粗多糖和精制多糖对DPPH、O_2^-·、·OH和NO_2-的清除作用,并与阳性对照VC进行比较,考察葛根多糖的抗氧化效果。结果葛根粗多糖中多糖含量为3.67%,精制多糖中多糖含量为8.74%。在0.5~4.0mg/mL浓度范围内,葛根粗多糖和精制多糖对DPPH、O_2^-·、·OH和NO_2^-均有不同程度的清除作用,清除活性顺序为·OH>O_2^-·>NO_2^->DPPH,且清除效果与多糖浓度呈剂量-效应关系,但清除率均低于阳性对照VC。在清除自由基时,相同浓度的葛根粗多糖的清除能力强于精制多糖。结论葛根多糖具有良好的抗氧化活性,可开发成新型的葛根源保健品或天然绿色的食品抗氧化剂。

Objective To explore antioxidative effects of polysaccharides of Puerariae Radix in vitro. Methods The polysaccharides were extracted from Puerariae Radix using ultrasonic extraction technology and detected by phenol-sulfuric acid method. Compared with the VC positive control, the scavenging activity of crude and refined polysaccharides of Puerariae Radix were studied, including DPPH, O2 ^- ·, ·OH and NO2^ - . Results The polysaccharide contents in crude and refined extracts were 3.67% and 8.74%, respectively. The results showed that the crude and refined polysaccharides of Puerariae Radix had scavenging capacity in different degrees on the DPPH, O2^-·, ·OH and NO2^-, and the order of scavenging activity was ·OH〉O2-·〉NO2-〉DPPH. In the range of 0.5～4.0 mg/mL, the scavenging effects were in a dose-effect relationship with the concentration of polysaccharides. The scavenging ability against 4 kinds of free radicals of crude polysaccharides was stronger than that of refined polysaccharides from Puerariae Radix in the same concentration. Antioxidative effects of the polysaccharides were weaker than those of the VC positive control. Conclusion The polysaccharides from Puerariae Radix have good anti-oxidation ability which can be developed into a kind of new health product or natural green food antioxidant.