霉菌和酵母菌显色纸片效果初步评价

Primary evaluation of count plate in the detection of yeast and mold

目的初步评价新研发显色纸片对霉菌和酵母菌检验效果。方法用本实验室研制的霉菌和酵母菌显色纸片(简称HK),与国外3M同类产品及传统国家标准计数培养基沙氏葡萄糖琼脂(SDA),对霉菌和酵母菌的检验效果从特异性、选择性和生长率3个方面进行对比测试。结果对7株酵母菌,HK的纸片有更好的显色效果,HK纸片对全部7株酵母菌均有显色反应,而3M对6株显色明显,1株显色不明显;对17株霉菌,HK对其中16株显色效果明显,3M对其中15株霉菌显色效果明显。对23株非目标菌2种纸片均能良好抑制。2种纸片的生长率差异无统计学意义(P=0.399),对各种菌的生长率均达到0.9以上。结论用新研发的显色纸片检测霉菌和酵母菌,特别是对酵母菌,具有比较好的效果。

Objective To evaluate the detection efficacy of count plate for yeast and mold. Methods To compare the count plate made in our labortory（ HK） with the similar product made by 3M and SDA on specificity,selectivity and growth rate in the detection of mold and yeast. Results HK was better than 3M in the test of 7 yeast strains with better chromogenic effect,which had reaction to all the 7 strains. 3M had chromogenic effect to 6 strains of yeast,with no significant effect to 1 strain. In the test of 17 mold strains,HK had good chromogenic effect to 16 strains,3M had good chromogenic effect to 15 strains. In the test of23 non-target bacteria strains,the two count plate bothes had good inhibition. There was no statistical significant on the difference of growth rate between the 2 count plates（ P = 0. 399）. All growth rates were higher than 0. 9. Conclusion The yeast and mold count plate made in our laboratory has a good effect in detecting mould and yeast,especially for the yeast.