变应性鼻炎患者外周血嗜酸粒细胞及IL-33水平的表达

Expression of eosinophils and IL-33 levels in peripheral blood of patients with allergic rhinitis

目的:探讨变应性鼻炎(AR)患者外周血嗜酸粒细胞(EOS)计数与IL-33水平的关系及未经任何治疗的AR患者(A组)、经皮下免疫治疗至少1年后AR患者(B组)、经皮下免疫治疗至少1年后伴变应性哮喘(AA)的AR患者(C组)这3组的IL-33水平、嗜酸粒细胞计数、视觉模拟评分(VAS)的关系。方法:选取20例健康者作为对照组参与实验,所有受试者行血细胞分析,统计嗜酸粒细胞数量。采用酶联免疫(ELISA)检测外周血IL-33水平,所得数据用GraphPad.Prism 5.0与SPSS 22.0处理。AR患者行VAS评分并评估鼻部症状。结果:A组IL-33水平高于其他组IL-33水平(P<0.05);B组IL-33水平与C组IL-33水平相比,差异无统计学意义(P>0.05);B组和C组IL-33水平均高于对照组IL-33水平(P<0.05);A组嗜酸粒细胞计数高于其他组,各组间相互比较嗜酸粒细胞计数差异无统计学意义(P>0.05);A组VAS评分高于B组VAS评分(P<0.05);A组VAS评分与C组VAS评分比较差异无统计学意义(P>0.05);B组VAS评分与C组VAS评分比较差异无统计学意义(P>0.05)。经皮下免疫治疗(SCIT)至少1年后AR患者自觉鼻塞、流清涕、打喷嚏等症状均有明显减轻。Spearman检验AR患者IL-33水平与嗜酸粒细胞计数呈弱正相关(P<0.05,r=0.287)。结论:SCIT对AR疗效明显,可缓解患者临床症状,还可降低患者IL-33水平和外周血嗜酸粒细胞计数。

Objective：To investigate the relationship between the count of eosinophils（EOS）in peripheral blood and the serum levels of IL-33,and to discuss the relations among serum levels of IL-33,the count of EOS,visual analog scale（VAS）in different groups.Method：According to different treatments,the patients are divided into three groups：the untreated allergic rhinitis（AR）group（group A）,the AR group who had been treated subcutaneous imunotherapy（SCIT）for at least a year（group B）and the AR complicated with allergic asthma group who had been treated subcutaneous imunotherapy（SCIT）for at least a year（gourp C）.All subjects were conducted blood cell analysis,and EOS were counted.The serum levels of IL-33 were measured by enzyme-linked immune（ELISA）,and the obtained date were analysed by GraphPad.Prism 5.0 and SPSS 22.0.AR patients were asked to fill out VAS and were assessed nasal symptoms.Result：The serum levels of IL-33 in the group A were higher than that in other subjects（P〈0.05）.The serum levels of IL-33 in the group B showed no significant difference between the group B and the group C（P〈0.05）.The serum levels of IL-33 in the group B were higher than that in the control group（P〈0.05）.The serum levels of IL-33 in the group C were higher than that in the control group（P〈0.05）.The count of EOS in the group A were higher than that in other subjects,and there is no difference between with each other（P〈0.05）.The VAS in the group A were higher than that in the group B（P〈0.05）and there is no significant difference between the group A and the group C（P〉0.05）.There is no difference between the group B and the group C（P〉0.05）.After at least one-year SCIT,the symptoms of AR patients were obviously relieved,such as consciously rhinobyon,rhinorrhea,sneezing and so on.Spearman test showed the serum levels of IL-33 in the AR patients has a weak correlation with the count of eosinophils（P〈0.05,r=0.287）.Conclusion：SCIT is an effective treatment for AR patients.role on AR,which can alleviate the symptoms of patients,also can reduce the levels of IL-33 and the count of EOS in peripheral blood.