羟基红花黄色素A对脓毒症小鼠外周血促炎/抗炎因子的影响

Effect of Hydroxysafflor Yellow A on Pro/Anti-Inflammatory Cytokines in Peripheral Blood with Sepsis in Mice

【目的】观察羟基红花黄色素A(HSYA)对脓毒症小鼠外周血炎症介质的影响,探讨其治疗脓毒症的作用。【方法】将NIH小鼠随机分为对照组、假手术组、脓毒症模型组、HSYA组,每组24只。采用盲肠结扎穿孔法(CLP)制备脓毒症小鼠模型,分别于CLP术前12 h、手术时、术后12 h尾静脉给予HSYA120 mg/kg/次,对照组、假手术组、模型组给予等体积生理盐水注射液。观察动物一般情况改变,检测血常规、血生化及血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子(TNF-α)的含量及mRNA表达水平,培养外周血细菌负荷量。【结果】术后24 h,模型组动物出现竖毛、肛门残留粪便等;与对照组、假手术组、HSYA组相比,模型组小鼠全血白细胞计数(WBC)、血小板水平(PLT)明显下降,血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、尿素氮(BUN)、IL-6、IL-10、TNF-α含量及mRNA表达明显升高,细菌负荷量明显增多,差异有统计学意义(P<0.05)。【结论】HSYA对脓毒症小鼠具有治疗作用,可减少脓毒症时细菌入血,减轻炎症介质导致的损伤,抑制亢进的炎症反应。

【Objective】To explore the effect of HSYA on pro/anti-inflammatory cytokine′s levels and mRNA expression in peripheral blood of mice.with sepsis.【Methods】Dividing NIH mice into four groups,as normal group,sham group,CLP group and HSYA group,24 mice in each group. The CLP sepsis mouse model was established. HSYA（120 mg/kg）were injected intravenously at 12 h before the operation,and 0 h and 12 h following CLP,and other groups were given normal saline. observed the animals behavior changes,measured the levels WBC,PLT,ALT,AST,BUN in serum,detected the levels and mRNA expression of IL-6,IL-10,TNF-α in peripheral blood. cultured of peripheral blood bacteria loads.【Results】24 h after surgery,mice in CLP group appeared furring,feces residues on anus etc. compared to normal group,sham group and HSYA group,WBC,PLT,ALT,AST,BUN,levels and mRNA expression of IL-6,IL-10,TNF-α showed significant increases,it was also found that bacterial load was significant increased in model group.【Conclusions】HSYA has a therapeutic effect in mice with sepsis,can reduce bacteria into the blood,and inhibit inflammatory mediators which caused tissue damage.