载脂蛋白A5基因多态性与冠心病患者血脂及冠脉狭窄程度的相关性

Association of APOA5 Polymorphisms with Plasma Lipid Levels and Severity of Coronary Stenosis in Patients with Coronary Heart Disease

【目的】探讨中国汉族人群载脂蛋白A5(APOA5)基因rs2075291和rs3135507多态性位点与冠心病(CHD)患者血脂水平及冠脉狭窄程度的关系。【方法】应用聚合酶链式反应-限制性片段长度多态性法(PCR-RFLP)对324例CHD患者和152例非CHD对照者进行rs2075291和rs3135507多态性分型并比较不同基因型之间血脂水平的差异。采用Gensini评分系统评价CHD患者的冠脉狭窄程度,并使用多因素线性回归法分析rs2075291和rs3135507多态性以及其他因素与Gensini得分之间的相关性。【结果】CHD组高血压患病率、甘油三酯(TG)、总胆固醇(TC)、载脂蛋白B100(APOB100)、脂蛋白a[Lp(a)]、TG/高密度脂蛋白胆固醇(HDL-C)、TC/HDL-C、低密度脂蛋白胆固醇(LDL-C)/HDL-C和APOB100/载脂蛋白AI(APOAI)水平高于对照组,HDL-C和APOAI水平低于对照组,差异均有统计学意义(P<0.05)。rs2075291和rs3135507多态性等位基因及基因型分布频率在CHD组与对照组之间差异无统计学意义。在对照组中,rs2075291多态性GT基因型TG和TG/HDL-C水平高于GG基因型,HDL-C水平低于GG基因型,差异均有统计学意义(P<0.05);在CHD组中,rs2075291多态性GT基因型TG/HDL-C和TC/HDL-C高于GG基因型,差异有统计学意义(P<0.05)。在CHD组中,rs3135507位点A等位基因携带者APOB100水平低于GG基因型,差异有统计学意义(P<0.05)。多因素回归分析结果显示,rs2075291和rs3135507多态性与Gensini得分的相关性无统计学意义(P>0.05)。【结论】APOA5 rs2075291影响血脂水平,但与CHD发生发展无明显相关性,此研究结果需大样本多中心的病例-对照研究加以证实。

【Objective】To explore the possible effects of the apolipoprotein A5（APOA5）gene rs2075291 and rs3135507 polymorphisms on plasma lipid levels and the severity of coronary stenosis in patients with coronary heart disease（CHD）in Chinese Han people.【Methods】Polymerase chain reaction-restriction fragment length polymorphism method（PCR-RFLP）was used to identify the genotypes of the rs2075291 or rs3135507 polymorphism for the 324 patients with CHD and the 152 CHD-free controls,and the lipid levels between the genotypes were compared. The severity of coronary stenosis was assessed by the Gensini scoring system,andthe associations of the rs2075291 and rs3135507 polymorphisms as well as other factors with the Gensini scores were analyzed by multivariate linear regression.【Results】The CHD patients had higher prevalence of hypertension,and higher levels of triglycerides（TG）,total cholesterol（TC）,apolipoprotein B100（APOB100）,lipoprotein（a）[Lp（a）],TG/high-density lipoprotein cholesterol（HDL-C）,TC/HDL-C,low-density lipoprotein cholesterol（LDL-C）/HDL-C and APOB100/apolipoprotein AI（APOAI）,and lower levels of HDL-C and APOAI than the controls（P〈0.05 for all）. The rs2075291 or rs3135507 allele and genotype frequencies were similar between the CHD group and the control group. The subjects with the rs2075291 GT genotype had higher levels of TG and TG/HDL-C,and lower levels of HDL-C than the subjects with the GG genotype in controls（P〈0.05 for all）. In CHD patients,those with the rs2075291 GT genotype had higher levels of TG/HDL-C and TC/HDL-C than the patients with the GG genotype（P〈0.05 for both）. Regarding the rs3135507 polymorphism,the A allele carriers had lower level of APOB100 than the patients with the GG genotype（P〈0.05）. No significant association between the rs2075291 or rs3135507 polymorphism and the Gensini scores were detected by multivariate linear regression（P〉0.05）.【Conclusion】The APOA5 rs2075291 polymorphism had significant effects on plasma lipid levels,but no effects on the occurrence and development of CHD. Further multicenter case-control studies with large samples are needed to validate these findings.