氯胺酮相关性膀胱炎大鼠逼尿肌热休克蛋白27的表达及其意义

Expressions and significances of heat shock protein 27 in detrusor tissue of rats with ketamine-associated cystitis

研究热休克蛋白27(HSP27)在氯胺酮诱导膀胱炎大鼠逼尿肌中的表达及临床意义.基于氯胺酮腹腔内注射诱导大鼠膀胱炎模型,选择不同时间通过尿流动力学检测膀胱收缩功能,收集大鼠膀胱组织,常规病理染色观察膀胱粘膜及逼尿肌结构,免疫组化法检测HSP27在逼尿肌中的表达水平.实验后观察结果表明,同对照组相比,实验组尿动力学表现为膀胱收缩频率显著增加及初感膀胱容量明显减小;对照组膀胱组织无明显病理及免疫表达变化,而实验组可观察到不同程度膀胱粘膜充血,炎症细胞浸润,HSP27表达水平的上升,且HSP27表达水平与诱导时间呈正相关.实验结果表明HSP27的表达水平可能与氯胺酮膀胱炎的逼尿肌收缩活动程度相关.

To investigate the expression of heat shock protein 27 （HSP27） in detrusor tissue of rats with ketamine and clinical significance. Rats were injected intraperitoneal with ketamine to establish the cystitis model. Through urodynamic detection the function of bladder contraction was studied, and then urinary bladders of the rats were harvested for routine pathological staining to analysis bladder mucosa and detrusor structure. HSP27 expression level in detrusor tissue at different time point was detected by immunohistochemical （IHC） staining. In comparison to the control group, urodynamic change of ketamine treated group showed significantly increased frequency of bladder contraction and reduced bladder capacity of first sensation; there is no significant change on pathological and immune expression in the control group, while it was observed in the experimental group with different degree of bladder mucosal hyperemia, inflammatory cells infiltration and elevated HSP27 expression levels; additionally, HSP27 expres- sion level was positively correlated with the induction time. These results suggested that the expression levels of HSP27 might have a correlation with the degree of detrusor contraction activities of ketamine-associated cystitis.