白藜芦醇对大鼠急性坐骨神经压迫损伤后运动功能及脊髓前角神经元的影响

Effects of resveratrol on motor function and anterior horn neuron of spinal cord following acute sciatic nerve compression injury in rats

目的探讨白藜芦醇（Resveratrol）对大鼠急性坐骨神经压迫损伤后运动功能及脊髓前角神经元的影响。方法将32只雄性SD大鼠随机分为四组，分别为：白藜芦醇组（Res）；二甲基亚砜组（DMSO）；生理盐水组（NS）；假手术组（Sham）。白藜芦醇组、二甲基亚砜组、生理盐水组建立急性坐骨神经压迫损伤模型后，分别连续给予白藜芦醇、二甲基亚砜、生理盐水腹腔注射10d；假手术组（Sham）大鼠仅暴露坐骨神经后予以缝合。分别于术前1d、术后第1、3、7、10天观察各组大鼠体重、脚趾伸展度和坐骨神经功能指数（SFI）的变化情况；于术后第10天取材，采用免疫荧光染色技术检测大鼠L4～L6段脊髓前角运动功能神经元细胞特异性核蛋白（NeuN）和微管相关蛋白2（MAP2）的表达。结果各组间大鼠体重变化差异无统计学意义；与假手术组相比，Res、DMSO和NS组大鼠患肢运动功能受损，其前角神经元损伤严重；大鼠脚趾伸展度及SFI值提示，在术后第3、7、10天，Res处理组均较DMSO组和NS组维持上调（P〈0．01）；在第10天，Res处理组大鼠腰段脊髓前角NeuN和MAP2的表达均高于DMSO组和NS组，同时其神经元细胞数量的减少得到了缓解。结论在大鼠急性坐骨神经损伤模型中，白藜芦醇有助于术侧肢体运动功能的恢复。

Objective To investigate the effects of resveratrol （Res） on motor function and the anterior horn neuron of lumbar spinal cord after acute sciatic nerve compression injury in rats. Methods The rat model with acute sciatic nerve compression injury was established in 32 Sprague Dawley （SD） rats, which were randomly divided into four groups：Res group, Dimethyl Sulfoxide （DMSO） group, Normal Saline （NS） group and sham-operation group. Res, DMSO and saline were successively injected by intraperitoneal for 10 days after established crush acute sciatic nerve compression injury model, while sham-operation group was sutured only after exposure to the sciatic nerve. The weight, the change of toe extension angle, and the sciatic functional index （SFI） of rats were observed at the 1st day before operation and the 1st,3rd ,Tth, and 10th days after surgery. The expressions of neuron-specific nuclear protein （NeuN） and microtubule-associate protein 2 （MAP2） were detected by immunofluorescent staining of I^-L6 spinal cord anterior horn on the 10th day after surgery. Results No significant changes were found in the weight of rats among four groups. Compared to the sham, the motor function of the injured limb in Res, DMSO, and NS rats was impaired, and the anterior horn neurons were seriously damaged. But the differences of the change of toe extension and the sciatic functional index of rats were significantly higher in Res group than that of the DMSO group and NS group （P 〈0. 01 ） at the 3rd, 7th, and 10th days after surgery. The expressions of NeuN and MAP2 in the anterior horn of rat lumbar spinal cord were up-regulated in Res group relative to DMSO and NS, and the number of neurons in the lumbar spinal cord was significantly relieved at the 10th days. Conclusions Res was significant to rat model of acute sciatic nerve injury, which could increase the number of neurons in the anterior horn of the spinal cord and help the recovery of motor function.