浊点萃取结合LC-MS/MS法测定野鸢尾黄素对气道炎症模型豚鼠体内花生四烯酸的影响

Determination of Arachidonic Acid in Guinea Pigs with Airway Inflammatory Model by Cloud Point Extraction Combined with LC-MS/MS Method

目的:建立气道炎症模型豚鼠血浆中花生四烯酸(AA)的测定方法,考察野鸢尾黄素降低气道炎性反应的作用。方法:以烟熏配合合胞病毒滴鼻制造气道炎症豚鼠模型,以AA-d8为内标,采用Triton-14对血浆样品进行浊点萃取,并采用LC-MS/MS法MRM监测模式测定豚鼠血浆中AA的浓度。结果:血浆中AA在0.1~100μg·m L-1范围内线性关系良好,日内精密度为2.53%~7.82%,日间精密度为10.51%~13.41%,提取回收率81.20%~90.77%。结论:该方法环保、准确、稳定,可用于豚鼠血浆中AA的测定。

Objective： To establish a method for the determination of arachidonic acid（ AA） in plasma of guinea pigs with airway inflammation model and investigate the effect of irigenin on reducing airway inflammatory response. Methods： The model of airway inflammation in guinea pigs was made by smokation combined with nasal infection. The concentration of AA in guinea pig plasma was determined by LC-MS/MS with MRM monitoring mode by using Triton-14 as the cloud point extraction and AA-d8 as internal standard. Results： The linearity of AA in the range of 0. 1-100 μg·m L-1was good. The inter-day precision was2. 53% ~ 7. 82%. The intra-day precision was 10. 51% ~ 13. 41%. The recovery was 81. 20% ~ 90. 77%. Conclusion： The method is environmentally friendly,accurate and stable and can be used for the determination of AA in guinea pig plasma.