紫锥菊地上部分4种酚酸类成分的HPLC法测定研究

Determination of Four Phenolic Acids in Above-ground Portion of Echinacea Purpurea by HPLC

目的:建立用高效液相色谱(HPLC)同时测定紫锥菊地上部分单咖啡酰酒石酸、咖啡酸、绿原酸和菊苣酸4种成分含量的方法。方法:采用HPLC法,色谱柱为ZORBAX SB-C_(18)(250 mm×4.6 mm,5μm);梯度洗脱,流动相为乙腈-0.1%磷酸溶液,流速1.0 m L·min^(-1);柱温35℃,检测波长为330 nm,进样量10μL。结果:4种酚酸类成分分离效果良好,单咖啡酰酒石酸、绿原酸、咖啡酸、菊苣酸分别在4.19~104.80μg/m L,0.81~20.24μg/m L,0.77~19.28μg/m L,13.20~330.00μg·m L^(-1)内线性关系良好;平均加样回收率在97.5%~101.6%。结论:本法准确、灵敏,重现性较好,可实现检控紫锥菊地上部分酚酸类成分的含量。

Objective： To establish a method by HPLC for simultaneously determinating contents of caftaric acid,chlorogenic acid,caffeic acid and chicory acid in the above-ground portion of Echinacea Purpurea. Methods： In the study,ZORBAX SB-C_（18） chromatographic column（ 250 mm × 4. 6 mm,5 μm） was used as a stationary phase and acetonitrile-0. 1% phosphoric acid aqueous was as mobile phase. In the gradient elution program,the flow rate was set as 1. 0 m L·min^-1,column temperature was 35℃,detection wavelength was 330 nm and sample quantity was 10 μL. Results： Four phenolic acids were separated well and effectively. The data showed a good linear relationship when the concentration range of caftaric acid,chlorogenic acid,caffeic acid and chicory acid were respectively 4. 19-104. 80 μg·m L^-1,0. 81-20. 24 μg·m L^-1,0. 77-19. 28 μg·m L^-1,66. 00-330. 00μg·m L^-1. The average recoveries were between 97. 5% and 101. 6%. Conclusion： The HPLC method was accurate,sensitive and has good reproducibility,which can realize to determinate and control the contents of phenolic acids in the above-ground portion of Echinacea Purpurea.