汉坦病毒感染乳大鼠大脑皮层星形胶质细胞（英文）

Hantavirus infection of astrocytes from cerebral cortex of newborn rats

目的建立汉坦病毒(HTNV)76-118株或汉城病毒(SEOV)L99株感染乳大鼠大脑皮层星形胶质细胞的体外培养体系,并检测其对汉坦病毒的易感性。方法通过免疫荧光显微技术、免疫印迹法、反转录聚合酶链反应检测星形胶质细胞对汉坦病毒的易感性,并通过反转录聚合酶链反应检测病毒S片段和GFAP基因表达情况。结果 HTNV或SEOV感染星形胶质细胞后病毒核衣壳蛋白(NP)和GFAP的表达增加,且GFAP和NP共定位,两者可能相互作用,同时病毒S片段和GFAP基因表达增加。结论 HTNV或SEOV能有效感染和激活星形胶质细胞,GFAP可能调节血脑屏障结构或功能的完整性、病毒NP合成及病毒复制。

Objective The primary focus of this research was to use astrocytes from newborn Sprague-Dawley rats less than 24 h infected with Hantaan 76-118 （HTNV） or Seoul virus （SEOV） strain L99 as an infection model in vitro to examine astrocyte susceptibility to hantavirus infection. Methods Astrocytes susceptibility to hantavirus infection was examined by immunofluorescence microscopy, Western blotting and genereverse transcription PCR （RT-PCR）. The expression of GFAP gene was detected by RT-PCR assay. Results HTNV or SEOV infection rapidly induced viral nucleocapsid protein （NP） and glial fibrillary acidic portein （GFAP） expressions in astrocytes. GFAP and NP in infected astrocytes were co-localized. Upregulation of S segment RNA and GFAP gene was confirmed. Conclusion Our findings indicate that HTNV or SEOV availably infects and activates astrocytes, and GFAP may be actively involved in regulating structural and functional integrity of the BBB,viral NP synthesis and replication.