羽裂报春苣苔的组织培养研究

Tissue Culture of Primulina pinnatifida

以羽裂报春苣苔的叶片为外植体,通过研究不同灭菌方法、不同植物生长调节剂种类和浓度对组织培养的影响,建立了羽裂报春苣苔的组织培养体系。结果表明:羽裂报春苣苔适宜的外植体灭菌方法为采用75%酒精灭菌20 s,无菌水冲洗3次;然后用0.1%升汞灭菌6 min,无菌水冲洗3次;再用0.1%升汞灭菌6 min,无菌水冲洗3次,成活率为55.40%。不定芽的诱导培养基为MS+6-BA 1.0~2.0 mg/L+NAA 0.01~0.10 mg/L,诱导率达100.00%。在适宜的增殖培养基MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+活性炭0.1 g/L上,增殖系数高达8.61;在生根培养基MS+IBA 0.5 mg/L+活性炭0.1 g/L上,生根率达100.00%,生根时间为19.10 d。移栽基质为泥炭∶珍珠岩∶河沙=4∶1∶1(V∶V∶V),移栽成活率为99.60%。

With the leaves of Primulina pinnatifida as the explants, the tissue culture technique system of P. pinnatifida was studied based on the effects of sterilization methods, plant growth regulator types and concentrations. The results showed that the suitable method for the sterilization of the explants was the leaves were sterilized in 75%alcohol for 20 seconds, then soaked in 0.1% Hg Cl2 for 6 minutes, and soaked in 0.1% Hg Cl2 for another 6minutes. The survival rate of the explants was 55.40%. The suitable culture medium for adventitious bud induction was MS＋6-BA 1.0-2.0 mg/L＋NAA 0.01-0.1 mg/L, the most suitable culture medium for adventitious bud multiplication was MS＋6-BA 2.0 mg/L＋NAA 0.05 mg/L＋activated carbon 0.1 g/L, and the multiplication coefficient was 8.61. The optimum culture medium for rooting culture was MS＋IBA 0.5 mg/L＋activated carbon 0.1 g/L, and the rooting rate was 100%. For the cultivation, the suitable ratio of transplanting medium of the in vitro plantlets was peat ∶ perlite ∶sand 4 ∶ 1 ∶ 1（V ∶ V ∶ V）, and the survival rate of transplanting of the in vitro plantlets reached 99.60%.