脊髓损伤后外周组织炎症反应在并发2型糖尿病中的作用研究

Study on the effect of inflammatory reactions of peripheral tissues after spinal cord injury on concurrent type 2 diabetes mellitus

目的通过观察SD大鼠脊髓损伤后血糖的变化及其外周组织炎症反应状态,探讨脊髓损伤并发2型糖尿病的可能机制。方法将24只SD大鼠随机分为两组:假模组和脊髓损伤组,其中假模组又分为假模普通饲料组(Sham+LFD)和假模三高饲料组(Sham+HFD)两个亚组,脊髓损伤组分为脊髓损伤普通饲料组(SCI+LFD)和脊髓损伤三高饲料组(SCI+HFD)两个亚组。以改良的Allen’s撞击法制作脊髓损伤动物模型,在实验开始前及8周时监测各组体重、血糖、葡萄糖耐量试验指标。于8周时取大鼠肝脏、脾脏及内脏脂肪组织,应用实时荧光定量PCR方法观察各组织胰岛素、TNF-α、IL-1β、IL-6及IL-10等炎症因子的表达情况。结果各组大鼠体重均有明显增长。脊髓损伤后大鼠血糖较损伤前明显升高,外周组织TNF-α、IL-1β、IL-6表达较假模组明显升高,IL-10则较假模组明显下降。相比Sham+LFD组,Sham+HFD组、SCI+LFD组以及SCI+HFD组大鼠组织中胰岛素、TNF-α、IL-1β含量显著增加,IL-10显著减少;相比Sham+HFD组,SCI+HFD组大鼠组织中胰岛素、TNF-α、IL-1β含量进一步增加,IL-10则进一步减少。结论脊髓损伤后外周炎症反应在脊髓损伤后并发2型糖尿病中有重要作用。

Objective To investigate the possible mechanism of spinal cord injury complicated with type 2 diabetes mellitus by observing the changes of blood glucose and the inflammatory response of peripheral tissue in SD rats after spinal cord injury. Methods A total of 24 SD rats were randomly divided into two groups： sham model group and spinal cord injury group. The sham model group was further divided into two subgroups： Sham＋LFD group and Sham＋HFD group. Spinal cord injury group was further divided into two groups： SCI＋LFD group and SCI＋HFD group. The animal model for spinal cord injury was made by modified Allen＇s impact method. Body weight, blood glucose and glucose tolerance indices were measured before experiment and 8 weeks after the experiment. At 8 weeks, the rat liver, spleen and visceral adipose tissues were collected. The expression of insulin and inflammatory factors such as TNF-α, IL-1β, IL-6 and IL-10 were observed by real-time fluorescence quantitative PCR. Results The body weight in each group was increased significantly. Blood glucose in rats after spinal cord injury was significantly higher than that before injury. The expression of TNF-α, IL-1β and IL-6 in peripheral tissues was significantly higher than that in the sham model group, and IL-10 was significantly lower than that in the sham model group. Compared with the Sham＋LFD group, the levels of insulin, TNF-α and IL-1β in Sham＋HFD group, SCI＋LFD group, and SCI＋HFD group were significantly increased, and IL-10 was significantly decreased. Compared with Sham＋HFD group, the levels of insulin, TNF-α and IL-1β in SCI＋HFD group were increased further, and IL-10 was further decreased. Conclusion Peripheral inflammatory response after spinal cord injury plays an important role in the treatment of concurrrent type 2 diabetes mellitus after spinal cord injury.