肝片形吸虫囊蚴抵抗力观察

Resistance on Fasciola hepatica metacercariae

目的观察肝片形吸虫囊蚴经加热、干燥、食用调料等处理后的存活情况。方法将肝片形吸虫囊蚴经1)加热:微波高火30s、60s;沸水10、30s和1、2、3min。2)干燥:室温下自然干燥24、40h。3)食源调料:蒜汁、酱油、醋以及3种等量混合液浸泡10、20、30min;38°白酒及饱和盐水各浸泡10、20、30min。4)4℃下保存10个月。结果 2种加热方式处理后囊蚴全部死亡;放置于玻片上的囊蚴干燥24h,死亡4只,附着于叶片上的囊蚴干燥40h,死亡2只;在蒜汁、酱油、醋中浸泡10、20、30min,囊蚴全部存活;在3种调料的等量混合液、饱和盐水及38°白酒中浸泡10、20、30min后,均有部分存活,另有一部分囊蚴结构清晰但虫体不动,但也无明显死囊蚴的特征;在4℃下保存10个月,仍有存活的囊蚴。结论常用的调料对囊蚴不起作用,自然干燥1~2d不能完全杀灭囊蚴,在低温潮湿环境中可较长时间保持活力。沸水加热10s或微波高火30s即可杀死囊蚴。

We observed the survival situation after treatments of Fasciola hepatica metacercariae by heating, drying and food seasoning. Results showed that after treating with microwave high fire and boiling water, all the metacercariae died. There were four metacercariae died after putting on the slide and dried them for 24 hours, while two died after attaching on the blade for 40 hours. The metacercariae all survived in the garlic, soy sauce, and vinegar liquid for 10, 20 and 30 mini After 10, 20 and 30 rain soaking in the same amount of mixed liquor of the three kinds of condiments, saturated brine and the liquor of 38%, part of the metacercariae were survival. The rest of the metacercariae showed clear structures, but the bodies did not move with no obvious characteristic of death of metacercariae. Storing for 4 ℃ in the next 10 months, there was still the survival of metacercariae. In conclusion, the commonly used seasoning had no effect on metacercariae. Natural drying for 1-2 days could not completely kill the metacercariae. A low temperature for a long time could maintain vitality. Heating via boiling water for 10 sec or microwave high fire for 30 s could kill metacercariae.