睾丸支持细胞对体外精子活力的影响

The effect of testicular sertoli cells on sperm motility in vitro

通过建立小鼠睾丸支持细胞与精子共培养的方法,达到体外提高精子活力的目的。利用睾丸组织切片技术,观察并分析支持细胞对生精细胞的作用。从6~7日龄小鼠睾丸中分离睾丸支持细胞,体外培养两天后,形成细胞单层作为滋养层。取小鼠附睾尾内精子,转移到以支持细胞为滋养层的培养液中培养,同时设立对照组。通过精子分析仪观察各组精子的活力,结果显示,以支持细胞为滋养层的精子前向运动百分比为56.1±5.81%,显著高于对照组40.7±2.96%(P<0.05)。利用激光共聚焦显微镜观察精子结构,结果显示两组精子形态结构差异不显著,表明支持细胞能够提高体外精子的活力,但不改变精子的形态结构。

In this study, the mouse testicular Sertoli cells were established as a trophoblast, through the method that sperm co--cultured with Sertoli cells to increase sperm motility. The effects of sertoli cells on spermatogenic cells were observed and analyzed, through the testieular tissue test. The testicular Sertoli cells were isolated from the testes of 6-7 days old mice and cultured in vitro for two days to form cell monolayers. The spermatozoa in the mouse epididymis were removed and then were transferred to a cul ture medium that Sertoli cells as trophoblasts, at the same time set up control group, the sperm motility of each group was observed by sperm analyzer. The research shows that, the forward movement percentage of spermatozoa co- cultured with Sertoli cells as trophoblast was 56. 1±5.81%, significantly higher than that of the control group 40.7±2.96% （P〈0.05）, this method greatly improved the vitality of spermato zoa. Laser confocal experimental results show that the sperm morphology of experimental group was not significant different from that of control group, indicating that Sertoli ceils improved sperm motility in vitro, but did not change the sperm morphology.