蕨菜中原蕨苷含量的测定及加工过程对原蕨苷含量的影响

Determination of ptaquiloside in Pteridium aquilinum by HPLC and the effect of ptaquiloside during processing

建立蕨菜及其制品中原蕨苷的HPLC检测方法,探讨加工工艺对蕨菜中原蕨苷含量的影响。蕨菜及其制品液氮研磨后,以体积分数30%甲醇作为提取溶剂,按固液比5∶40(g∶mL)室温下涡旋振荡提取30 min,提取液经聚酰胺6柱净化后,在碱性和酸性环境下将原蕨苷转化为稳定性较强的蕨素B,通过HPLC法检测蕨素B的含量来计算样品中原蕨苷含量,并以此方法考察热烫和热风干燥加工对蕨菜中原蕨苷含量的影响。在该色谱条件下线性范围为1~10μg/mL,r=0.999,线性良好,对蕨素B的回收率为92.3%~102.4%,RSD均小于5.00%。蕨菜经热烫加工后原蕨苷含量显著降低,经热风干燥后原蕨苷含量却显著升高。该方法灵敏度高、定量准确、重现性好,可用于蕨菜及其制品中原蕨苷含量的检测,热烫加工可有效降低蕨菜中原蕨苷的含量。

To establish a method for determination ptaquiloside in Pteridium aquilinum by HPLC, and evaluate the effect of ptaquiloside during processing. Method： Bracken and its products were grinded in liquid nitrogen and extracted 30min at room temperature with 30% methanol with solid-liquid ratio of 5 g： 40 mL. The extract was purified by polyamide column, and was converted into pterosin B in alkaline and acidic environment; by detecting the content of pterosin B using HPLC, the content of ptaquiloside was calculated. The content of ptaquiloside was used in the comparison of two drying method： hot water bleaching and hot air drying. Result： The method can effectively extract and purify the ptaquiloside in bracken fern and can convert all the ptaquiloside into the pterosin B. The linear range was 1-10μg/mL, r=0. 999 9. The recovery rate of ptaquiloside was between 92.3% and 102.4% and RSD was less than 5.00%. The content of ptaquiloside in bracken fern significantly decreased by blanching processing, and signifi- cantly increased by hot air drying processing. Conclusion： This method has good sensitivity, accuracy, and repeatability. It can be used for the evaluation of the content of ptaquiloside in bracken fern. The content of ptaquiloside in bracken fern could be effectively reduced by blanching processing.