硝酸盐诱导蛋白与谷子抗旱性的关系研究

The preliminary study about the relationship between nitrate-induced protein and drought tolerance in foxtail millet

[目的]硝酸盐诱导蛋白(NOI)是一类重要的抗逆蛋白,在多种植物中发挥作用。谷子作为重要的旱作植物,在农作物生产中占有重要地位,探究谷子中NOI与抗旱性的关系,对发掘谷子抗旱相关基因具有重要意义。[方法]运用生物信息学方法,对谷子中编码NOI的基因进行分析,并在PEG干旱胁迫下,探究抗旱品种勾勾母鸡咀(GG)和干旱敏感品种晋汾16(JF16)中编码硝酸盐诱导蛋白(NOI)的基因的相对表达情况。[结果]编码NOI的基因的启动子中存在与干旱相关的响应元件。在PEG干旱胁迫处理与非胁迫处理条件下,勾勾母鸡咀(GG)和晋汾16(JF16)中编码NOI的基因的表达模式差异显著。[结论]基于上述分析,推测硝酸盐诱导蛋白(NOI)可能参与谷子的抗旱调节,为深入研究谷子抗旱机理提供了理论基础和依据。

[Objective]Nitrate-induced protein（NOI）is an important protein related to stress and works in variety of plants.As an important drought-tolerance crop,foxtail millet plays a significant role in crop production,so it is important sense to explore the connection between NOI and drought tolerance and the unique genetic resources.[Methods]Using bioinformatics,we analysed the genes coding NOI and studied the relative expression level in the drought-tolerant variety Go Go Mu Ji Ju（GG）and the drought sensitive variety Jin Fen 16（JF16）.[Results]The results showed that there were response elements associated with drought response in the gene＇s protein promoter.Under PEG stress,Go Go Mu Ji Ju（GG）and Jin Fen 16（JF16）,showed a strikingly different expression pattern.[Conclusion]Based on the analysis above,we speculate that NOI could be involved in the regulation of drought tolerance in foxtail millet and have a different expression in varieties of foxtail millet.The study could provide a theoretical basis and foundation for further study of drought tolerance in foxtail millet.