马身猪ZNF280D基因新型剪接体的鉴定及生物信息学分析

Identification and bioinformatics analysis of alternative splicing isoforms of ZNF280D gene in Mashen Pig

[目的]本试验旨在探究马身猪中锌指蛋白(zinc finger protein)ZNF280D基因的剪接体类型。[方法]在猪转录组测序对ZNF280D基因剪切位置预测的基础上,采用RT-PCR和克隆测序技术对预测的剪切位点进行验证,检测该基因不同剪接体的结构并进行生物信息学分析。[结果]共检测到2个剪接体,分别是ZNF280D1和ZNF280D2,ZNF280D1为该基因的常规转录本,ZNF280D2第8外显子5'端大部分缺失。ZNF280D1含有3个C2H2型锌指结构域,属tC2H2型锌指蛋白,ZNF280 D2含有4个C2H2型锌指结构域,属maC2H2型锌指蛋白。[结论]ZNF280D2与DNA的亲和力大于ZNF280D1。

[Objective]In this study,the splice variant of the gene of zinc finger protein 280D（ZNF280D）in Mashen pigs was investigated.[Methods]Based on the prediction of the ZNF280 D gene splicing position in the pig transcriptome sequence,these splice sites were verified by RT-PCR amplification,cloning and sequencing technique.[Results]Two alternative splicing isoforms of the ZNF280 D gene called ZNF280D1 and ZNF280D2 were discoveried in Mashen pigs.Compared with ZNF280D1,the exon 8in ZNF280D2 was missed.Three C2H2 zinc finger domain structures were found in ZNF280D1 that was a tC2H2 type zinc finger protein.Four C2H2 zinc finger domain structures were found in ZNF280D2 that was a maC2H2 type zinc finger protein.[Conclusion]The greater affinity for DNA was detected in ZNF280D2.