两种试剂盒检测儿童肺炎支原体免疫球蛋白M的比较分析

Comparative analysis of two kits in detecting immunglobulin M in children mycoplasma pneumonia

目的比较两种试剂盒检测方法对儿童肺炎支原体感染的检出情况,为临床检验提供科学依据。方法分别用蛋白质生物芯片法和免疫胶体金法,对156例疑似肺炎支原体感染患儿血清IgM进行平行检测,同时与临床诊断结果比较,分析2种方法的诊断效果。结果 156例患儿中,临床诊断肺炎支原体感染阳性117例,免疫胶体金法48例阳性,阳性率30.8%,诊断符合率41.0%,蛋白质生物芯片法89例阳性,阳性率57.1%,诊断符合率76.1%。2种方法比较差异有统计学意义(P<0.05)。结论蛋白质生物芯片法检测肺炎支原体Ig M的特异性和敏感性均高于胶体金法。免疫胶体金法可用于肺炎支原体初筛试验,蛋白质芯片法可用于临床早期快速检测肺炎支原体。

Objective To compare the detection of Mycoplasma pneumoniae infection in children by two kits, and to provide scientific basis for clinical examination. Methods The serum immunglobulin M （IgM） levels in 156 children with suspected Mycoplasma pneumoniae infection were detected by protein biochip method and immune colloidal gold method. Results In 156 cases, 117 cases were positive by the clinical diagnosis of Mycoplasma pneumoniae infection, immune colloidal gold method was positive in 48 cases, the positive rate was 30.8%, the diagnostic coincidence rate was 41.0%, the protein biochip method was positive in 89 cases, the positive rate was 57.1%, the coincidence rate of diagnosis was 76.1%. The two methods were tested, and the difference was statistically significant （P〈0.05）. Conclusion The specificity and sensitivity of detection of Mycoplasma pneumoniae IgM by protein biochip method were higher than that of colloidal gold method. Colloidal gold method can be used for mycoplasma pneumoniae screening test, the Protein microarray method can be used in the early detection of mycoplasma pneumoniae